Immunohistochemical analysis of in vivo patterns of expression of CPP32 (Caspase-3), a cell death protease.
Immunohistochemical analysis of in vivo patterns of expression of CPP32 (Caspase-3), a cell death protease.
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发表时间：
1997-04
期刊：
影响因子：
11.2
通讯作者：
M. Krajewska;H. G. Wang;S. Krajewski;J. Zapata;A. Shabaik;R. Gascoyne;John Calvin Reed
中科院分区：
文献类型：
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作者：
M. Krajewska;H. G. Wang;S. Krajewski;J. Zapata;A. Shabaik;R. Gascoyne;John Calvin Reed
CPP 32（Caspase-3）基因表达的体内模式，确定使用免疫组织化学方法和石蜡包埋的正常人体组织。针对重组人CPP 32蛋白产生兔多克隆抗血清，并且通过对各种人组织和细胞系的免疫印迹分析显示其具有特异性。CPP 32免疫反应性被选择性地发现在某些细胞类型，通常存在于胞质内，虽然偶尔细胞也包含核免疫染色。CPP 32免疫染色容易在例如表皮角质形成细胞、软骨软骨细胞、骨骨细胞、心肌细胞、血管平滑肌细胞、支气管上皮、肝细胞、胸腺细胞、浆细胞、肾小管上皮、精原细胞、前列腺分泌上皮细胞、子宫内膜和子宫肌层、乳腺导管上皮细胞以及胃、肠和结肠的胃肠上皮中检测到。与此相反，很少或没有观察到CPP 32免疫反应的内皮细胞，肺泡肺细胞，肾小球，乳腺肌上皮细胞，雪旺氏细胞，和大多数类型的脑和脊髓神经元。与CPP 32在凋亡性细胞死亡中的作用一致，在一些较短寿命类型的细胞中与较长寿命类型的细胞相比，注意到CPP 32免疫染色的相对强度的明显差异，包括（a）淋巴结、脾和扁桃体的次级滤泡内的生发中心（高）与套区（低）B淋巴细胞;（B）骨髓中成熟嗜中性粒细胞（高）对骨髓祖细胞（低）;（c）卵巢中黄体细胞（高）对卵泡颗粒细胞（低）;和（d）前列腺分泌上皮细胞（高）对基底细胞（低）。这些发现首次建立了白细胞介素-1 β转换酶/CED-3（Caspase）家族蛋白酶表达的细胞类型和分化特异性模式。
The in vivo patterns of CPP32 (Caspase-3) gene expression were determined using an immunohistochemical approach and paraffin-embedded normal human tissues. A rabbit polyclonal antiserum was generated against recombinant human CPP32 protein and shown to be specific by immunoblot analysis of various human tissues and cell lines. CPP32 immunoreactivity was selectively found in certain cell types and was typically present within the cytosol, although occasional cells also contained nuclear immunostaining. CPP32 immunostaining was easily detected, for example, in epidermal keratinocyes, cartilage chondrocytes, bone osteocytes, heart myocardiocytes, vascular smooth muscle cells, bronchial epithelium, hepatocytes, thymocytes, plasma cells, renal tubule epithelium, spermatogonia, prostatic secretory epithelial cells, uterine endometrium and myometrium, mammary ductal epithelial cells, and the gastrointestinal epithelium of the stomach, intestine, and colon. In contrast, little or no CPP32 immunoreactivity was observed in endothelial cells, alveolar pneumocytes, kidney glomeruli, mammary myoepithelial cells, Schwann cells, and most types of brain and spinal cord neurons. Consistent with a role for CPP32 in apoptotic cell death, clear differences in the relative intensity of CPP32 immunostaining were noted in some shorter-lived types of cells compared to longer-lived, including (a) germinal center (high) versus mantle zone (low) B lymphocytes within the secondary follicles of lymph nodes, spleen, and tonsils; (b) mature neutrophils (high) versus myeloid progenitor cells (low) in bone marrow; (c) corpus luteal cells (high) versus follicular granulosa cells (low) in the ovary; and (d) prostate secretory epithelial cells (high) versus basal cells (low). These findings establish for the first time the cell type- and differentiation-specific patterns of expression of an interleukin-1beta converting enzyme/CED-3 (Caspase) family protease.