Thermally sensitive conductive hydrogel using amphiphilic crosslinker self-assembled carbon nanotube to enhance neurite outgrowth and promote spinal cord regeneration
Thermally sensitive conductive hydrogel using amphiphilic crosslinker self-assembled carbon nanotube to enhance neurite outgrowth and promote spinal cord regeneration
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DOI：
10.1039/c5ra20780k
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发表时间：
2016-03
期刊：
影响因子：
3.9
通讯作者：
Li Sang;Yuqing Liu;Wenxi Hua;Kaige Xu;Guobao Wang;W. Zhong;Leyu Wang;Shuchai Xu;M. Xing;Xiaozhong Qiu
中科院分区：
文献类型：
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作者：
Li Sang;Yuqing Liu;Wenxi Hua;Kaige Xu;Guobao Wang;W. Zhong;Leyu Wang;Shuchai Xu;M. Xing;Xiaozhong Qiu
脊髓损伤会导致人体严重的感觉或运动损伤。人们已经通过尝试物理和生化策略来努力激活神经功能。碳纳米管作为导电材料，除了为神经元提供细胞外支架外，还被用来传输电信号，以改善细胞间的通讯和串扰。我们报道了一种热敏水凝胶，采用正异丙基丙烯酰胺、聚乙二醇二丙烯酸酯-十二胺-1-(2-氨基乙基)哌嗪(PEGDA-DD-AEP)的低聚两亲性交联剂和单壁碳纳米管的共聚。我们假设凝胶中的碳纳米管可以改善神经突生长和神经再生。为了克服碳纳米管的聚集问题，利用两亲交联剂的疏水链来稳定碳纳米管。碳纳米管-聚（正异丙基丙烯酰胺）（PNIPAAM）水凝胶是可注射的并提高了导电性。我们发现水凝胶可能具有促进 SH-SY5Y 细胞生长的潜力，在给予电刺激时神经突明显生长。在脊髓损伤模型中，在C7处创建1毫米×1毫米×1毫米的空腔，我们发现水凝胶促进神经组织再生并减少疤痕组织的形成。因此，水凝胶可能是用于神经元网络重建和脊髓再生的潜在修复生物材料。
Spinal cord injury leads to severe sensory or motor damage in the human body. Efforts have been made to activate the nerve function by trying physical and biochemical strategies. Carbon nanotubes as conductive materials have been used to transmit electrical signals to improve cell–cell communication and cross-talk, besides providing an extracellular scaffold for neurons. We reported a thermally sensitive hydrogel using copolymerization of n-isopropylacrylamide, the oligomeric amphiphilic crosslinker of polyethylene glycol diacrylate–dodecylamine–1-(2-aminoethyl)piperazine (PEGDA–DD–AEP), and single-walled carbon nanotubes. We hypothesized that carbon nanotubes in the gel could improve neurite outgrowth and nerve regeneration. In order to overcome the aggregation issue of carbon nanotubes, the hydrophobic chains of the amphiphilic crosslinker were used to stabilize the nanotubes. The carbon nanotube–poly(n-isopropylacrylamide) (PNIPAAM) hydrogel was injectable and improved the electrical conductivity. We found that the hydrogel may have potential to promote the growth of SH-SY5Y cells, with significant neurite outgrowth while electrical stimulation was given. In a spinal cord injury model, creating a 1 mm × 1 mm × 1 mm cavity at C7, we found that the hydrogel promoted nerve tissue regeneration and reduced the formation of scar tissue. Therefore, the hydrogel may be a potential repairing biomaterial for neuron network reconstruction and spinal cord regeneration.