Changes in community structure of methanogenic archaea brought about by water-saving practice in paddy field soil
Changes in community structure of methanogenic archaea brought about by water-saving practice in paddy field soil
复制标题
DOI：
10.1016/j.soilbio.2012.11.022
复制
发表时间：
2013-03
影响因子：
9.7
通讯作者：
Takeshi Watanabe;Y. Hosen;R. Agbisit;L. Llorca;N. Katayanagi;S. Asakawa;M. Kimura
中科院分区：
文献类型：
--
作者：
Takeshi Watanabe;Y. Hosen;R. Agbisit;L. Llorca;N. Katayanagi;S. Asakawa;M. Kimura
采用聚合酶链反应-变性梯度凝胶电泳（PCR- dgge）和针对古菌16S rRNA基因和产甲烷古菌mcrA基因的定量PCR分析，揭示了水田土壤中产甲烷古菌群落结构在干湿交替节水（AWD）条件下的变化。在连续淹水（CF）和AWD节水处理下，在15 cm深度土壤水势降至- 20 kPa时进行灌溉，共采集4次土壤样品。群落分析在2008年雨季（2008WS）和2009年旱季（2009DS）和2009年雨季（2009WS）三个水稻栽培期进行。PCR-DGGE分析显示，古菌16S rRNA基因因水分管理的不同而不同。在3个水稻栽培时期，CF样地群落随时间逐渐变化，而AWD样地群落变化较大。主成分分析和所选16S rRNA基因测序结果表明，在CF区以Methanosarcinales成员为主，而在AWD区以Methanocellales成员和未培养的“Thaumarchaeota”组为特征。古菌16S rRNA和产甲烷古菌mcrA基因数量在CF和AWD地块间差异显著，但在2009年ds和2009年ws间差异不显著。因此，AWD节水措施并未减少产甲烷古菌的数量，但对稻田土壤中产甲烷古菌的群落结构有一定影响。
Changes in the community structure of methanogenic archaea in a paddy field soil brought about by an alternate wetting and drying (AWD) water-saving practice were shown by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and quantitative PCR analyses targeting archaeal 16S rRNA gene and methanogenic archaeal mcrA gene. Soil samples were collected four times from the field plots under the treatments of continuous flooding (CF) and AWD water-saving, where irrigation was conducted when soil water potential at 15 cm depth decreased to −20 kPa. The community analysis was performed over three rice cultivation periods in the wet season in 2008 (2008WS) and the dry and wet seasons in 2009 (2009DS and 2009WS, respectively). PCR-DGGE analysis showed that the archaeal 16S rRNA genes differed depending on water management. During the three rice cultivation periods, the community of the CF plots gradually changed with time, while the community of the AWD plots possessed a relatively large variation. Principal component analysis and sequencing analysis of selected 16S rRNA genes indicated that members of Methanosarcinales dominated in the CF plots while members of Methanocellales and uncultured group of “Thaumarchaeota” characterized the AWD plots. Numbers of archaeal 16S rRNA and methanogenic archaeal mcrA genes were significantly different between the CF and AWD plots in 2008WS, but not in 2009DS and 2009WS. Thus, the AWD water-saving practice did not reduce populations of the methanogenic archaea but moderately influenced the community structure in the paddy field soil.