Incorporation of aligned PCL-PEG nanofibers into porous chitosan scaffolds improved the orientation of collagen fibers in regenerated periodontium
Incorporation of aligned PCL-PEG nanofibers into porous chitosan scaffolds improved the orientation of collagen fibers in regenerated periodontium
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DOI：
10.1016/j.actbio.2015.07.023
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发表时间：
2015-10-01
影响因子：
9.7
通讯作者：
Zhou, Shaobing
中科院分区：
文献类型：
--
作者：
Jiang, Wenlu;Li, Long;Zhou, Shaobing
牙周膜（PDL）是一组高度排列和组织化的结缔组织纤维，介于牙根表面和牙槽骨之间。这种独特的结构对于牙周组织的特定生理功能是必不可少的。牙周组织的再生已被广泛研究，但很少有人关注牙周膜纤维的排列及其功能。在这项研究中，我们制作了一个三维多层支架植入高度对齐的可生物降解的聚（ε-己内酯）-聚（乙二醇）（PCE）共聚物电纺纳米纤维垫到多孔壳聚糖（CHI）提供地形线索，并引导牙周组织的定向再生。与随机组和多孔对照组相比，定向纳米纤维包埋支架可引导细胞定向排列和伸长，促进细胞的浸润、存活和牙周韧带相关基因的表达。在体内，与随机组和多孔对照组相比，定向纳米纤维包埋支架显示再生PDL更有组织地排列，几乎垂直于牙根表面，形成更广泛的成熟胶原纤维。排列支架组再生牙周组织中periostin的表达水平更高，支持牙齿的矿化组织形成更明显。将排列的PCE纳米纤维并入多孔CHI中被证明适用于牙周组织的定向再生，这可能进一步用于具有专门方向的各种人体组织的再生。重要性声明牙周组织的再生已经被研究人员广泛研究，但很少有人关注牙周韧带（PDL）纤维的排列及其功能。关键的问题是提供指导的方向细胞与排列的胶原纤维垂直对牙根表面。本研究旨在通过模拟支架结构促进牙周组织定向再生。进一步评价支架的体内外性能，以测试其局部导向和牙周组织愈合的潜力。我们还认为，我们的研究可能会为各种人体组织的再生提供一个专门的方向。(C)2015 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The periodontal ligament (PDL) is a group of highly aligned and organized connective tissue fibers that intervenes between the root surface and the alveolar bone. The unique architecture is essential for the specific physiological functionalities of periodontium. The regeneration of periodontium has been extensively studied by researchers, but very few of them pay attention to the alignment of PDL fibers as well as its functionalities. In this study, we fabricated a three-dimensional multilayered scaffold by embedding highly aligned biodegradable poly (s-caprolactone)-poly(ethylene glycol) (PCE) copolymer electrospun nanofibrous mats into porous chitosan (CHI) to provide topographic cues and guide the oriented regeneration of periodontal tissue. In vitro, compared with random group and porous control, aligned nanofibers embedded scaffold could guide oriented arrangement and elongation of cells with promoted infiltration, viability and increased periodontal ligament-related genes expression. In vivo, aligned nanofibers embedded scaffold showed more organized arrangement of regenerated PDL nearly perpendicular against the root surface with more extensive formation of mature collagen fibers than random group and porous control. Moreover, higher expression level of periostin and more significant formation of tooth-supporting mineralized tissue were presented in the regenerated periodontium of aligned scaffold group. Incorporation of aligned PCE nanofibers into porous CHI proved to be applicable for oriented regeneration of periodontium, which might be further utilized in regeneration of a wide variety of human tissues with a specialized direction.Statement of SignificanceThe regeneration of periodontium has been extensively studied by researchers, but very few of them give attention to the alignment of periodontal ligament (PDL) fibers as well as its functionalities. The key issue is to provide guidance to the orientation of cells with aligned arrangement of collagen fibers perpendicular against the root surface. This study aimed to promote oriented regeneration of periodontium by structural mimicking of scaffolds. The in vitro and in vivo performances of the scaffolds were further evaluated to test the topographic-guiding and periodontium healing potentials. We also think our research may provide ideas in regeneration of a wide variety of human tissues with a specialized direction. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.