Evidence of Brucella infection in marine mammals in the North Atlantic Ocean
Evidence of Brucella infection in marine mammals in the North Atlantic Ocean
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北大西洋海洋哺乳动物感染布鲁氏菌的证据
DOI：
10.1136/vr.144.21.588
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发表时间：
1999
影响因子：
2.2
通讯作者：
J. Godfroid
中科院分区：
文献类型：
--
作者：
M. Tryland;L. Kleivane;Á. Alfredsson;M. Kjeld;A. Árnason;S. Stuen;J. Godfroid
1983年至1996年期间，在冰岛西部的沃茨、马延西北部浮冰区、挪威北方海岸和科拉半岛、斯瓦尔巴特群岛西部的沃茨和巴伦支海共采集了4种海豹和3种鲸鱼的1 386份血清样本;用间接ELISA（蛋白G缀合物）检测它们是否存在抗布鲁氏菌抗体。用经典的布鲁氏菌病血清学试验，如血清凝集试验、EDTA改良血清凝集试验、虎红试验和补体结合试验以及抗补体ELISA对阳性血清进行复检。除须海豹外，所有被调查的物种均检测到抗布鲁氏菌抗体（Erignathus spanatus），有以下流行：冠海豹（Cystophora cristata）35%;格陵兰海豹（Phoca groenlandica）2%;环斑海豹（Phoca hispida）10%;小须鲸（尖吻长须鲸）8%;长须鲸（Balaenoptera physalus）11%;从一头血清反应阳性的小须鲸的肝脏和脾脏中分离出一种属于布鲁氏菌属的分离物。研究结果表明，针对布鲁氏菌属物种表面脂多糖的抗体广泛分布于北大西洋的海洋哺乳动物中。
Between 1983 and 1996 a total of 1386 samples of serum were taken from four species of seal and three species of whale in the waters west of Iceland, the area of pack-ice north-west of Jan Mayen, the northern coast of Norway and the Kola Peninsula, the waters west of Svalbard, and the Barents Sea; they were tested for the presence of anti-Brucella antibodies with an indirect ELISA (protein G conjugate). The positive sera were re-tested with classical brucellosis serological tests, such as the serum agglutination test, the EDTAmodified serum agglutination test, the Rose Bengal test, and the complement fixation test, as well as an anti-complement ELISA. Anti-Brucella antibodies were detected in all the species investigated, except for the bearded seal (Erignathus barbatus), with the following prevalences: hooded seals (Cystophora cristata) 35 per cent; harp seals (Phoca groenlandica) 2 per cent; ringed seals (Phoca hispida) 10 per cent; minke whales (Balaenoptera acutorostrata) 8 per cent; fin whales (Balaenoptera physalus) 11 per cent; and sei whales (Balaenoptera borealis) 14 per cent. An isolate belonging to the genus Brucella was obtained from the liver and spleen of one of the seropositive minke whales. The findings suggest that antibodies against the surface lipopolysaccharide of Brucella species are widely distributed among marine mammals in the North Atlantic Ocean.