Comprehensive DNA microarray analysis of Bacillus subtilis two-component regulatory systems
Comprehensive DNA microarray analysis of Bacillus subtilis two-component regulatory systems
复制标题
DOI：
10.1128/jb.183.24.7365-7370.2001
复制
发表时间：
2001-12-01
影响因子：
3.2
通讯作者：
Fujita, Y
中科院分区：
文献类型：
--
作者：
Kobayashi, K;Ogura, M;Fujita, Y
最近，通过对枯草芽孢杆菌双组分调控系统（DegS-DegU， ComP-ComA和PhoR-PhoP）的DNA微阵列分析表明，在同源传感器激酶缺乏的背景下，双组分系统的反应调节因子的过量产生会影响基因的调控，包括其靶基因。通过DNA芯片分析揭示了过量生产对基因表达的全基因组影响。在目前的工作中，我们通过这种策略新分析了24个双组分系统，留下了8个不太可能适用的系统。这一分析揭示了这些双组分系统的各种靶基因候选。特别值得注意的是，在几个双组分系统之间似乎发生了有趣的相互作用。此外，从一些未知的双组分系统的候选靶基因列表中推断出它们的可能功能。这项工作是启发式的，但为进一步深入研究枯草芽孢杆菌双组分调控系统提供了有价值的信息。在这项工作中获得的DNA微阵列数据可在KEGG表达数据库网站（http://www.genome.ad.jp/kegg/expression）上获得。
It has recently been shown through DNA microarray analysis of Bacillus subtilis two-component regulatory systems (DegS-DegU, ComP-ComA, and PhoR-PhoP) that overproduction of a response regulator of the two-component systems in the background of a deficiency of its cognate sensor kinase affects the regulation of genes, including its target ones. The genome-wide effect on gene expression caused by the overproduction was revealed by DNA microarray analysis. In the present work we newly analyzed 24 two-component systems by means of this strategy, leaving out 8 systems to which it was unlikely to be applicable. This analysis revealed various target gene candidates for these two-component systems. It is especially notable that interesting interactions appeared to take place between several two-component systems. Moreover, the probable functions of some unknown two-component systems were deduced from the list of their target gene candidates. This work is heuristic but provides valuable information for further study toward a comprehensive understanding of the B. subtilis two-component regulatory systems. The DNA microarray data obtained in this work are available at the KEGG Expression Database website (http://www.genome.ad.jp/kegg/expression).