Gap Junction Coupling Shapes the Encoding of Light in the Developing Retina
Gap Junction Coupling Shapes the Encoding of Light in the Developing Retina
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DOI：
10.1016/j.cub.2019.10.025
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发表时间：
2019-12-02
期刊：
影响因子：
9.2
通讯作者：
Feller, Marla B.
中科院分区：
文献类型：
--
作者：
Caval-Holme, Franklin;Feller, Marla B.
在常规光感受器成熟之前，发育中的视网膜中的环境照明的检测由固有光敏视网膜神经节细胞（ipRGC）介导，并且对于驱动若干生理过程（包括光厌恶、瞳孔光反射和昼夜节律的光诱导）是关键的。ipRGC在这些早期年龄编码环境光强度变化的策略尚不清楚。使用双光子钙响应的无监督聚类，然后检查解剖特征，我们发现新生儿视网膜的群体活动可以建模为六个功能组，其由ipRGC亚型和非ipRGC细胞类型的混合物组成。通过结合成像、全细胞记录、药理学和解剖学技术，我们发现细胞类型的功能混合部分由间隙连接偶联介导。总之，这些数据表明，细胞自主的内在光响应和ipRGC之间的间隙连接耦合都有助于发育中的视网膜对光强度的正确编码。
Detection of ambient illumination in the developing retina prior to maturation of conventional photoreceptors is mediated by intrinsically photosensitive retinal ganglion cells (ipRGCs) and is critical for driving several physiological processes, including light aversion, pupillary light reflexes, and photoentrainment of circadian rhythms. The strategies by which ipRGCs encode variations in ambient light intensity at these early ages are not known. Using unsupervised clustering of two-photon calcium responses followed by inspection of anatomical features, we found that the population activity of the neonatal retina could be modeled as six functional groups that were composed of mixtures of ipRGC subtypes and non-ipRGC cell types. By combining imaging, whole-cell recording, pharmacology, and anatomical techniques, we found that functional mixing of cell types is mediated in part by gap junction coupling. Together, these data show that both cell-autonomous intrinsic light responses and gap junction coupling among ipRGCs contribute to the proper encoding of light intensity in the developing retina.