Hormone-induced redistribution of calcium-activated phospholipid-dependent protein kinase in pituitary gonadotrophs.
Hormone-induced redistribution of calcium-activated phospholipid-dependent protein kinase in pituitary gonadotrophs.
复制标题
激素诱导的垂体促性腺激素中钙激活磷脂依赖性蛋白激酶的重新分布。
DOI：
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发表时间：
1985
影响因子：
4.8
通讯作者：
K. Catt
中科院分区：
文献类型：
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作者：
K. Hirota;T. Hirota;G. Aguilera;K. Catt
钙激活，磷脂依赖性蛋白激酶（蛋白激酶C）之间的细胞质和膜组分的分布进行了分析，在培养的垂体促性腺激素释放激素（GnRH）治疗过程中。在离心淘洗纯化的垂体细胞中，GnRH刺激期间蛋白激酶C再分布的程度与促性腺激素细胞的富集相关。促性腺激素释放激素（LH）从促性腺激素富集细胞刺激释放伴随着快速和剂量依赖性的细胞溶质蛋白激酶C的减少和相应的颗粒部分蛋白激酶C活性的增加。视黄酸直接抑制胞浆蛋白激酶C的活性，也减弱促性腺激素释放激素促性腺激素释放激素。这些发现，以及GnRH引起胞质蛋白激酶C快速易位为膜相关形式的能力，表明蛋白激酶C的激素激活是GnRH刺激垂体LH分泌的中间步骤。
The distribution of calcium-activated, phospholipid-dependent protein kinase (protein kinase C) between cytosol and membrane fractions was analyzed in cultured pituitary gonadotrophs during treatment with gonadotropin-releasing hormone (GnRH). In pituitary cells purified by centrifugal elutriation, the extent of protein kinase C redistribution during GnRH stimulation was correlated with the enrichment of gonadotrophs. GnRH-stimulated release of luteinizing hormone (LH) from gonadotroph-enriched cells was accompanied by a rapid and dose-dependent decrease in cytosolic protein kinase C and by a corresponding increase in protein kinase C activity in the particulate fraction. Retinal directly inhibited the activity of cytosolic protein kinase C and also attenuated the release of LH from GnRH-stimulated gonadotrophs. These findings, and the ability of GnRH to cause rapid translocation of cytosolic protein kinase C to a membrane-associated form, suggest that hormonal activation of protein kinase C is an intermediate step in the stimulation of pituitary LH secretion by GnRH.