Structure and evolution of 4-coumarate:coenzyme A ligase (4CL) gene families
Structure and evolution of 4-coumarate:coenzyme A ligase (4CL) gene families
复制标题
DOI：
10.1515/bc.2001.076
复制
发表时间：
2001-04-01
影响因子：
3.7
通讯作者：
Douglas, CJ
中科院分区：
文献类型：
--
作者：
Cukovic, D;Ehlting, J;Douglas, CJ
苯丙酸酶4-香豆酸辅酶A连接酶（4CL）在一般的苯丙酸代谢中起关键作用，4CL与一类更大的原核和真核腺苷酸形成酶有关，并与这些酶共享几个保守的肽基序。为了更好地表征杨树、欧芹和烟草4CL基因家族的性质，我们利用退化引物扩增了这些物种的4CL序列。在每个物种中，还发现了不同的4CL基因。获得了两个杨树4CL新基因的完整cDNA克隆，从而检测了它们的表达模式，并确定了木质部特异性异构体的底物利用谱。这些基因和基因片段的系统发育分析证实了先前的结果，表明4CL蛋白在进化上属于两个古老的亚群。对腺苷酸形成超家族中酶的系统发育比较分析表明，4cl、荧光素酶和醋酸辅酶A连接酶在超家族中形成不同的分支。根据本研究的分析，从拟南芥基因组计划中鉴定出的4个拟南芥4CL样基因与真正的4CL只有远亲关系，或者与脂肪酸辅酶a连接酶的关系更近，这表明先前鉴定的3个拟南芥4CL基因代表了该物种4CL基因家族的范围。
The phenylpropanoid enzyme 4-coumarate:coenzyme A ligase (4CL) plays a key role in general phenylpropanoid metabolism, 4CL is related to a larger class of prokaryotic and eukaryotic adenylate-forming enzymes and shares several conserved peptide motifs with these enzymes. In order to better characterize the nature of 4CL gene families in poplar, parsley, and tobacco, we used degenerate primers to amplify 4CL sequences from these species. In each species additional, divergent 4CL genes were found. Complete cDNA clones for the two new poplar 4CL genes were obtained, allowing examination of their expression patterns and determination of the substrate utilization profile of a xylem-specific isoform. Phylogenetic analysis of these genes and gene fragments confirmed previous results showing that 4CL proteins fall into two evolutionarily ancient subgroups. A comparative phylogenetic analysis of enzymes in the adenylate-forming superfamily showed that 4CLs, luciferases, and acetate CoA ligases each form distinct clades within the superfamily. According to this analysis, four Arabidopsis 4CL-like genes identified from the Arabidopsis Genome Project are only distantly related to bona fide 4CLs or are more closely related to fatty acid CoA ligases, suggesting that the three Arabidopsis 4CL genes previously characterized represent the extent of the 4CL gene family in this species.