Serum peptidome patterns for early screening of esophageal squamous cell carcinoma
Serum peptidome patterns for early screening of esophageal squamous cell carcinoma
复制标题
DOI：
10.1002/bab.1024
复制
发表时间：
2012-07-01
影响因子：
2.8
通讯作者：
Qiao, Liang
中科院分区：
文献类型：
--
作者：
Fan, Nai-Jun;Gao, Chun-Fang;Qiao, Liang
食管鳞状细胞癌是世界上最常见的恶性肿瘤之一。早期诊断是指导食管鳞癌治疗的关键。本研究的目的是确定血清肽组模式诊断食管鳞癌。为了鉴定用于诊断ESCC的新的肽组模式，对来自31名健康志愿者和32名ESCC患者的血清进行比较蛋白质组学分析，使用ClinProtTM试剂盒结合质谱法（MS）。这种方法使得能够确定可以区分ESCC血清和健康志愿者血清的肽组模式。为了进一步验证，肽组模式的诊断和鉴别诊断能力通过使用由来自31名ESCC患者、33名健康志愿者、38名结肠直肠患者和36名胃癌患者的血清组成的独立血清组进行盲法验证。使用快速分类器算法构建肽组模式，用于从对照样品中鉴定ESCC。使用质荷比为759、786、1，866、3，316和6，634的5个鉴别峰以几乎100%的准确度构建肽组模式。此外，肽组模式也可以区分验证组，具有较高的准确性。这些结果表明，ClinProt（TM）试剂盒与MS结合可显著提高ESCC诊断和鉴别诊断的准确性。
Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers in the world. Early diagnosis is critical for guiding the therapeutic management of ESCC. The present study aims to determine serum peptidome patterns for diagnosing ESCC. To identify novel peptidome patterns for diagnosing ESCC, sera from 31 healthy volunteers and 32 ESCC patients were subjected to a comparative proteomic analysis using a ClinProt (TM) Kit combined with mass spectrometry (MS). This approach enables the determination of peptidome patterns that can differentiate between ESCC sera and sera from healthy volunteers. For further validation, the diagnostic and differential diagnostic capabilities of the peptidome patterns were verified blindly by using an independent group of sera, consisting of sera from 31 ESCC patients, 33 healthy volunteers, 38 colorectal patients, and 36 gastric cancer patients. A Quick Classifier Algorithm was used to construct the peptidome patterns for the identification of ESCC from the control samples. Five of the identified peaks at mass to charge ratios 759, 786, 1,866, 3,316, and 6,634 were used to construct the peptidome patterns with almost 100% accuracy. Furthermore, the peptidome patterns could also differentiate the validation group with high accuracy. These results suggest that the ClinProt (TM) Kit combined with MS achieves significantly high accuracy for ESCC diagnosis and differential diagnosis.