The use of antisense approaches to study development.
The use of antisense approaches to study development.
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使用反义方法来研究发育。
DOI：
10.1002/dvg.1020140402
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Erickson,RP
中科院分区：
文献类型：
--
作者：
Erickson,RP
反义RNA或DNA有可能实现对基因表达的特定控制。DNA的信息含量包含在一条链中，通常被称为正义链，读作5‘到3’。反义RNA或DNA是指编码在相反链上且方向相反的互补序列，即碱基对仍然以5‘到3’方向共价连接，但互补链是反平行的。然而，相邻的基因可能编码在第一个基因的反义链上，即相反的方向。当然，这种互补的反义链具有与正义链杂交的能力。正是这种反义信息与它的补体杂交，被一些研究人员用来控制基因表达。这种利用反义RNA、DNA或修饰的核酸衍生物来干扰基因表达的方法已经蓬勃发展。在这一方法的发展历史中可以找到许多线索。Itoh和Tomizawa(1980)首次将反义RNA的存在描述为ColEL质粒复制中的一种自然的基因控制方法。在细菌和质粒调节中自然使用反义RNA的许多其他例子已经被发现。也许最新的，也是最优雅的研究之一，是舒斯特实验室对控制P1噬菌体溶原性的研究(Biere等人，1992)。在这个系统中，它是围绕核糖体结合位点的两对短序列的互补，这两个短序列参与了抑制物合成的控制。
Antisense RNA or DNA has the potential to allow the specific control of gene expression. The informational content of DNA is contained in one strand, usually called the sense strand, which is read 5’to 3’. Antisense RNA or DNA refers to the complementary sequence encoded on the opposite strand and running in the opposite direction ie the base pairs are still covalently linked in the 5’to 3’orientation but the complementary strand is antiparallel. An adjacent gene may, however, be coded on the first gene’s antisense strand, ie be oppositely oriented. This complementary, antisense strand, of course, has the ability to hybridize to the sense strand. It is the hybridization of this antisense information to its complement which is being used by a number of investigators to control gene expression.This use of antisense RNA, DNA, or modified nucleic acid derivatives to disrupt gene expression has burgeoned. Many threads may be found in the history of the development of this approach. Itoh and Tomizawa (1980) first described the presence of an antisense RNA as a natural method of gene control in ColEl plasmid replication. Many other examples of the natural use of antisense RNA in bacterial and plasmid regulation have been found. Perhaps the most recent, and one of the most elegant, is the Schuster lab study of the control of lysogeny of P1 phage (Biere et aE., 1992). In this system it is the complementarity of two pairs of short sequences encompassing the ribosome binding sites which are involved in the control of antirepressor synthesis.