Spo13 regulates cohesin cleavage.
Spo13 regulates cohesin cleavage.
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Spo13 调节粘连蛋白裂解。
DOI：
10.1101/gad.989302
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Prinz,Susanne
中科院分区：
文献类型：
--
作者：
Lee,BrianH;Amon,Angelika;Prinz,Susanne
减数分裂染色体分离的一个关键方面是内聚蛋白，一种将姐妹染色单体结合在一起的蛋白质复合体，在减数分裂I期间从染色体臂分离，在减数分裂II期间从着丝粒区分离。出芽酵母蛋白Spo13在防止减数分裂期间着丝粒凝聚蛋白丢失中起关键作用。我们已经确定了在有丝分裂细胞周期中Spo13过表达时获得中期停滞的分子基础。spo13过表达通过至少两种机制抑制晚期发病。首先，Spo13导致后期抑制剂Pds1降解的短暂延迟。其次，Spo13通过分离酶Esp1抑制内聚蛋白亚基Scc1/Mcd1或其减数分裂特异性同源物Rec8的裂解。发现Spo13不能阻止另一种Esp1底物Slk19的切割，这表明Spo13的过表达足以通过保护特定底物免受分离酶活性的影响来阻止内聚蛋白的切割。
A key aspect of meiotic chromosome segregation is that cohesin, the protein complex that holds sister chromatids together, dissociates from chromosome arms during meiosis I and from centromeric regions during meiosis II. The budding yeast protein Spo13 plays a key role in preventing centromeric cohesin from being lost during meiosis I. We have determined the molecular basis for the metaphase arrest obtained whenSPO13is overexpressed during the mitotic cell cycle. Overexpression ofSPO13inhibits anaphase onset by at least two mechanisms. First, Spo13 causes a transient delay in degradation of the anaphase inhibitor Pds1. Second, Spo13 inhibits cleavage of the cohesin subunit Scc1/Mcd1 or its meiosis-specific homolog, Rec8, by the separase Esp1. The finding that Spo13 did not prevent cleavage of another Esp1 substrate, Slk19, suggests that overexpression ofSPO13is sufficient to prevent cohesin cleavage by protecting specific substrates from separase activity.