Duplication of the ribosomal gene cluster in the marine polychaete Platynereis dumerilii correlates with ITS polymorphism
Duplication of the ribosomal gene cluster in the marine polychaete Platynereis dumerilii correlates with ITS polymorphism
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DOI：
10.1017/s002531540705566x
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发表时间：
2007-04-01
影响因子：
1.2
通讯作者：
Ferrier, David E. K.
中科院分区：
文献类型：
--
作者：
Hui, Jerome H. L.;Kortchagina, Natalia;Ferrier, David E. K.
杜氏扁丝藻是一种新兴的比较发育生物学和生态毒理学模式系统。因此，能够绘制基因和标记在其基因组中的位置将是非常有价值的，以便了解基因组如何变化或已经变化，无论是由于化学和环境损伤还是在进化过程中。在这里，我们报告的适应染色体荧光技术在硅。图杂交（FISH）的定位单拷贝基因的杜氏疟原虫。此外，重复的核糖体基因簇，这种环节动物是更可靠地揭示与FISH比以前的技术的银染色。这种重复的核糖体簇与内转录间隔区（ITS）的多态性相关，这对在系统发育或种群生物学工作中使用这些区域具有意义。
Platynereis dumerilii is an emerging model system for both comparative developmental biology and ecotoxicology. As such it would be extremely valuable to be able to map the location of genes and markers in its genome, in order to understand how the genome changes or has changed, either due to chemical and environmental insults or during evolution. Here we report the adaptation of the technique of chromosomal fluorescence in si. tu hybridization (FISH) to P. dumerlii for the localization of single-copy genes. Also the duplicated ribosomal gene cluster of this annelid is more reliably revealed with FISH than with the previous technique of silver staining. This duplicated ribosomal cluster correlates with polymorphisms in the internal transcribed spacer (ITS) regions, which has implications for the use of these regions in phylogenetic or population biology work.