GROWTH-RELATED COORDINATE REGULATION OF THE EARLY N-GLYCOSYLATION GENES IN YEAST
GROWTH-RELATED COORDINATE REGULATION OF THE EARLY N-GLYCOSYLATION GENES IN YEAST
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DOI：
10.1093/glycob/4.4.437
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发表时间：
1994-08-01
期刊：
影响因子：
4.3
通讯作者：
LENNON, K
中科院分区：
文献类型：
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作者：
KUKURUZINSKA, MA;LENNON, K
酿酒酵母的ALG7、ALG1和ALG2基因，其产物在蛋白n -糖基化的醇醇途径早期起作用，对细胞活力至关重要，其表达的扰动会导致G(1)特异性细胞周期阻滞。本研究表明，在酵母生命周期的G(0)/G(1)过渡点，ALG7、ALG1和ALG2基因的表达受到协调调节。碳饥饿诱导细胞从有丝分裂周期的G(1)阶段进入G(0)阶段，导致早期ALG基因mrna水平的时间依赖性下降。因此，葡萄糖的加入刺激G(0)阻滞细胞恢复增殖，导致其mrna的快速诱导。单独的环己亚胺也能诱导早期ALG转录本，尽管程度远低于葡萄糖。同时添加葡萄糖和环己亚胺引起这些mrna的超诱导，表明超过一个控制水平参与了它们的激活。与此一致的是，在环己亚胺的存在下，ALG7、ALG1和ALG2 mrna的快速降解被完全消除。这些数据表明，在酵母中，早期n -糖基化基因的调节方式与早期生长反应基因的调节方式相似。
The Saccharomyces cerevisiae ALG7, ALG1 and ALG2 genes, whose products function early in the dolichol pathway of protein N-glycosylation, are essential for cell viability, and perturbation in their expression causes G(1)-specific cell cycle arrest. Here, we show that expression of the ALG7, ALG1 and ALG2 genes is coordinately regulated at the G(0)/G(1) transition point in the yeast life cycle. Carbon starvation, which induces cells to exit from the G(1) stage of the mitotic cycle into G(0), resulted in a time-dependent decrease in the levels of the early ALG genes' mRNAs. Accordingly, addition of glucose, which stimulates the G(0)-arrested cells to resume proliferation, resulted in a rapid induction of their mRNAs. Cycloheximide alone also induced the early ALG transcripts, albeit to a much lower extent than glucose. Simultaneous addition of glucose and cycloheximide caused superinduction of these mRNAs, indicating that more than one control level was involved in their activation. Consistent with this, rapid degradation of ALG7, ALG1 and ALG2 mRNAs was completely abolished in the presence of cycloheximide. These data suggest that in yeast, the early N-glycosylation genes are regulated ina manner similar to that of the early growth-response genes.