Detection of Salmonella spp., Yersinia enterocolitica and verocytotoxin-producing Escherichia coli O157 in pigs at slaughter in Italy
Detection of Salmonella spp., Yersinia enterocolitica and verocytotoxin-producing Escherichia coli O157 in pigs at slaughter in Italy
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DOI：
10.1016/s0168-1605(02)00504-4
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发表时间：
2003-08-15
影响因子：
5.4
通讯作者：
Morabito, S
中科院分区：
文献类型：
--
作者：
Bonardi, S;Brindani, F;Morabito, S
1999年12月至2000年12月，在北方意大利的两个大型屠宰场随机选择了150头猪。收集盲肠材料和尸体拭子，并检查沙门氏菌、小肠结肠炎耶尔森氏菌和大肠杆菌O 157。检查扁桃体中的沙门氏菌和小肠结肠炎Y菌。沙门氏菌分离肠内容物的55（36.7%）标本，从8（5.3%）扁桃体，从9（6.0%）尸体。检测到10种不同的血清型;较常见的是德比沙门氏菌（37.8%），布雷德尼沙门氏菌（21.6%）和鼠伤寒沙门氏菌（14.8%）。S. DT 104型和DT 208型分别占45%和27.3%，U 302型占18.2%，无法分型的占9.1%。在6头（4.0%）屠宰猪的肠道物质和22头（14.7%）扁桃体中检测到小肠结肠炎Y菌，但在尸体上未发现该病原体。大多数小肠结肠炎耶尔森氏菌分离株（82.1%）属于血清型0：3生物型4，1株（3.6%）属于血清型0：9，13%不属于任何已知生物型。产Verocytotoxin E.大肠杆菌（VTEC）O 157从1头（0.7%）屠宰猪的肠内容物和1头（0.7%）胴体中分离到，4份（2.7%）粪便样品含有E.大肠杆菌O 157菌株eae和VT基因均为阴性。(C)2002 Elsevier Science B. V.保留所有权利。
From December 1999 to December 2000, 150 pigs were randomly selected in two large abattoirs of northern Italy. Caecal material and carcass swabs were collected and examined for Salmonella, Yersinia enterocolitica, and Escherichia coli O157. Tonsils were examined for Salmonella and Y enterocolitica. Salmonella was isolated from the intestinal content of 55 (36.7%) specimens, from 8 (5.3%) tonsils, and from 9 (6.0%) carcasses. Ten different serotypes were detected; the more common were Salmonella derby (37.8%), Salmonella bredeney (21.6%), and Salmonella typhimurium (14.8%). S. typhimurium isolates that belonged to phage-types DT104 and DT208 were 45% and 27.3%, respectively; 18.2% belonged to U302 and 9.1% were nontypeable. Y enterocolitica was detected in the intestinal matter of 6 (4.0%) slaughtered pigs and in 22 (14.7%) tonsils; however, this pathogen was not found on carcasses. The majority of Y enterocolitica isolates (82.1%) belonged to serotype 0:3 biotype 4, one (3.6%) belonged to serotype 0:9, and 13% did not belong to any known biotype. Verocytotoxin-producing E. coli (VTEC) O157 was isolated from the intestinal content of one (0.7%) slaughtered pig and from one (0.7%) carcass; four (2.7%) faecal samples contained E. coli O157 strains negative for the presence of both eae and VT genes. (C) 2002 Elsevier Science B.V. All rights reserved.