Immunogenicity of a Trivalent Recombinant Vaccine Against Clostridium perfringens Alpha, Beta, and Epsilon Toxins in Farm Ruminants.
Immunogenicity of a Trivalent Recombinant Vaccine Against Clostridium perfringens Alpha, Beta, and Epsilon Toxins in Farm Ruminants.
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DOI：
10.1038/srep22816
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发表时间：
2016-03-23
影响因子：
4.6
通讯作者：
Conceição FR
中科院分区：
文献类型：
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作者：
Moreira GM;Salvarani FM;da Cunha CE;Mendonça M;Moreira ÂN;Gonçalves LA;Pires PS;Lobato FC;Conceição FR
产气荚膜梭菌是一种产生多种毒素的厌氧细菌。其中，α，β和β毒素是导致最严重的C。家畜产气荚膜杆菌相关疾病。控制这些疾病的最好方法是接种疫苗。然而，市售疫苗是基于灭活毒素的，并且具有许多生产缺陷，这些缺陷可以通过使用重组抗原来克服。在这项研究中，我们在大肠杆菌中生产重组α，β和β毒素，以配制三价疫苗。通过在家兔中进行的效价试验评价了其有效性，其中疫苗产生了9.6、24.4和25.0 IU/mL针对相应毒素的中和抗体。此后，牛、绵羊和山羊接受相同的制剂，分别产生5.19 ± 0.48、4.34 ± 0.43和4.70 ± 0.58 IU/mL的α毒素，13.71 ± 1.17 IU/mL（对于所有三种物种）对β毒素的抑制率为12.74 ± 1.70、7.66 ± 1.69和8.91 ± 2.14 IU/mL。这些水平高于国际议定书建议的最低水平。因此，我们的疫苗在产生保护性抗体方面是有效的，因此，可能代表预防C.产气荚膜杆菌相关中毒的农场动物。
Clostridium perfringens is an anaerobic bacterium that produces several toxins. Of these, the alpha, beta, and epsilon toxins are responsible for causing the most severe C. perfringens-related diseases in farm animals. The best way to control these diseases is through vaccination. However, commercially available vaccines are based on inactivated toxins and have many production drawbacks, which can be overcome through the use of recombinant antigens. In this study, we produced recombinant alpha, beta, and epsilon toxins in Escherichia coli to formulate a trivalent vaccine. Its effectiveness was evaluated through a potency test in rabbits, in which the vaccine generated 9.6, 24.4, and 25.0 IU/mL of neutralizing antibodies against the respective toxins. Following this, cattle, sheep, and goats received the same formulation, generating, respectively, 5.19 ± 0.48, 4.34 ± 0.43, and 4.70 ± 0.58 IU/mL against alpha toxin, 13.71 ± 1.17 IU/mL (for all three species) against beta toxin, and 12.74 ± 1.70, 7.66 ± 1.69, and 8.91 ± 2.14 IU/mL against epsilon toxin. These levels were above the minimum recommended by international protocols. As such, our vaccine was effective in generating protective antibodies and, thus, may represent an interesting alternative for the prevention of C. perfringens-related intoxications in farm animals.