F-127-PEI co-delivering docetaxel and TFPI-2 plasmid for nasopharyngeal cancer therapy
F-127-PEI co-delivering docetaxel and TFPI-2 plasmid for nasopharyngeal cancer therapy
复制标题
F-127-PEI 共递送多西紫杉醇和 TFPI-2 质粒用于鼻咽癌治疗
DOI：
10.1016/j.msec.2015.12.049
复制
发表时间：
2016-04-01
影响因子：
7.9
通讯作者：
Nie, Guohui
中科院分区：
文献类型：
--
作者：
Liu, Tao;Zhang, Xinyu;Nie, Guohui
药物和基因的联合递送已成为肿瘤治疗的主要策略。然而，如何构建一种安全、载药量高、基因转染效率高的肿瘤共递送系统仍具有挑战性。本文合成了一种新型的可降解纳米载体F127-PEI，该载体由聚乙烯亚胺（PEI）连接的PEO-PPO-PEO（Pluronic F127）组成。将该纳米载体用于疏水性多西他赛（DOC）和功能性基因（TFPI-2质粒）的共递送治疗鼻咽癌（NPC）。结果表明，F127-PEI纳米载体具有较高的DOC负载量，具有良好的体外基因传递效果。联合给药分析表明，F127-PEI/DOC/TFPI-2复合物比单独使用DOC或TFPI-2更能诱导鼻咽癌HNE-1细胞凋亡，并能更明显地降低鼻咽癌HNE-1细胞的侵袭能力。此外，通过溶血和MTT测定，与PEI-25 k相比，F127-PEI共聚物表现出更好的血液相容性和更低的细胞毒性，这表明用于NPC治疗的有希望的潜力。(C)2015爱思唯尔B. V.保留所有权利。
The co-delivery of drug and gene has become the primary strategy in cancer therapy. However, to construct one safe co-delivering system with higher drug loading and gene transfection efficiency for cancer therapy is still challenging. Herein, a novel degradable nanocarriers were synthesized and characterized in this study, which was composed of polyethylenimine (PEI)-linked PEO-PPO-PEO (Pluronic F127), called F127-PEI. Then the nanocarrier was used for hydrophobic docetaxel (DOC) and functional gene (TFPI-2 plasmid) co-delivery to treat nasopharyngeal cancer (NPC). The results indicated that F127-PEI nanocarriers had higher DOC loading amount and possessed good gene delivery effect in vitro. For co-delivery analysis, the obtained F127-PEI/DOC/TFPI-2 complexes could induce a more significant apoptosis than DOC or TFPI-2 alone, and decreased invasive capacity of NPC HNE-1 cells more obviously. Moreover, the F127-PEI copolymer exhibited better blood compatibility and lower cytotoxicity compared to PEI-25k by the hemolysis and MTT assays, which suggests a promising potential for NPC therapy. (C) 2015 Elsevier B.V. All rights reserved.