Growth factors influence the sensitivity of leukemic stem cells to cytosine arabinoside in culture.
Growth factors influence the sensitivity of leukemic stem cells to cytosine arabinoside in culture.
复制标题
生长因子影响白血病干细胞对培养物中阿糖胞苷的敏感性。
DOI：
10.1182/blood.v73.5.1272.bloodjournal7351272
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发表时间：
1989
期刊：
影响因子：
20.3
通讯作者：
E. A. McCulloch
中科院分区：
文献类型：
--
作者：
J. Miyauchi;Colm A. Kelleher;C. Wang;Salomon Minkin;E. A. McCulloch
我们提出，急性髓细胞白血病 (AML) 中的原始细胞是由一小部分干细胞维持的更新群体。胚芽干细胞的自我更新和分化之间的平衡可能是有助于治疗结果的重要属性。阿糖胞苷 (ara-C) 包含在大多数治疗 AML 的化疗方案中。当构建 ara-C 存活曲线时，与依赖于末端分裂的程序相比，当使用主要检测自我更新分裂的测定法时，该药物似乎具有更大的毒性。 AML 母细胞在培养中通常对骨髓生成生长因子有反应；他们的反应通常包括自我更新、分化或两者兼而有之。 AML 原始细胞模型的这些特征导致预测生长因子将改变 ara-C 存活曲线，其方式取决于培养条件对自我更新和分化的影响。选择四个 AML 原始细胞群体来测试这一预测，这是基于我们通过添加或抑制一种或多种生长因子来操纵它们的能力。根据培养物中存在的生长因子，在 ara-C 存活曲线中观察到高度显着的变化，正如通过观察到的因子对更新和分化的影响所预测的。
We have proposed that the blasts in acute myeloblastic leukemia (AML) are renewal populations maintained by a small subpopulation of stem cells. The balance between self-renewal and differentiation in blast stem cells may be an important attribute contributing to treatment outcome. Cytosine arabinoside (ara-C) is included in most chemotherapeutic regimens for the treatment of AML. When ara-C survival curves are constructed, the drug appears to be more toxic when an assay is used that detects principally self-renewing divisions, compared with a procedure that depends on terminal divisions. AML blasts usually respond in culture to myelopoietic growth factors; their response often includes a change in self-renewal, differentiation, or both. These features of the model for AML blasts led to the prediction that growth factors would alter ara-C survival curves in a way that depended on the effects of the culture conditions on self-renewal and differentiation. Four AML blast populations were chosen to test this prediction on the basis of our ability to manipulate them by adding or withholding one or more growth factors. Highly significant changes were seen in the ara-C survival curves, depending on the growth factors present in the cultures as was predicted by the observed effects of the factors on renewal and differentiation.
影响因子：
56.9
作者：
SOUZA, LM;BOONE, TC;WELTE, K
通讯作者：
WELTE, K