Probing ND7/23 neuronal cells before and after differentiation with SERS using Sharp-tipped Au nanopyramid arrays
Probing ND7/23 neuronal cells before and after differentiation with SERS using Sharp-tipped Au nanopyramid arrays
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DOI：
10.1016/j.snb.2022.131724
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发表时间：
2022-03-24
影响因子：
8.4
通讯作者：
De Angelis, Francesco
中科院分区：
文献类型：
--
作者：
Iarossi, Marzia;Hubarevich, Aliaksandr;De Angelis, Francesco
局部等离子体纳米传感器的发展是敏感的，同时保持非侵入性和高细胞活力是具有挑战性的，但对细胞过程的研究有很大的兴趣。为此，我们开发了一种有效的SERS活性生物界面，通过主成分分析来监测细胞活性并区分未分化和分化的神经元。我们提出了一种等离子体顶端的金纳米金字塔（Au NPs）阵列，它受益于高纵横比和锋利的尖端，是优秀的SERS传感器。为了实现这种结构，我们开发了一种大规模廉价的制造路线，该路线基于带电纳米球的组装，利用胶体光刻技术作为掩膜，以更好地分隔和塑造纳米粒子。研究表明，尖端曲率为10 nm、局部等离子体共振波长为785 nm的Au NPs可以无损探测ND7/23神经元。我们证明，这些提示使我们能够在SERS底物上跟踪ND7/23神经元，检测膜成分、蛋白质和脂质，甚至细胞内DNA/RNA片段。
The development of local plasmonic nano sensors which are sensitive, whilst remaining non-invasive with high cell viability is challenging but of great interest for the investigation of cellular processes. With this aim, we developed an effective SERS active biointerface to monitor cell activity and discriminate between undifferentiated and differentiated neurons through principal component analysis. We propose a plasmonic tipped Au nanopyramids (Au NPs) array, which benefits from high aspect ratios and sharp tips that are excellent SERS sensors. To realise the structure, we developed a large-scale inexpensive fabrication route based on the assembly of charged nanospheres used as a mask by means of a colloidal lithography technique, to better space and shape the NPs. Here we show that Au NPs with tip curvatures of 10 nm and localized plasmon resonance at 785 nm can non-destructively probe ND7/23 neurons. We prove that these tips allow us to track ND7/23 neurons on the SERS substrate, detecting both the membrane constituents, proteins and lipids, and even intracellular DNA/RNA fragments.