Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation
Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation
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DOI：
10.1021/acsami.5b08314
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发表时间：
2015-11-04
影响因子：
9.5
通讯作者：
Ma, Dik-Lung
中科院分区：
文献类型：
--
作者：
Leung, Ka-Ho;He, Bingyong;Ma, Dik-Lung
我们报道了一个利用Ir（III）配合物、末端脱氧核苷酸转移酶（TdT）和结构折叠适体的金属离子、蛋白质和小分子的无标记、结构无关的发光传感平台。鉴定了一种新的g -四重体选择性Ir（III）配合物，以增强发光响应来检测新生的g -四重体基序。与文献中报道的大多数无标记DNA检测不同，该传感平台不需要适配体的特定二级结构，从而大大简化了DNA设计。该检测平台通过检测K+离子、凝血酶和可卡因作为金属离子、蛋白质和小分子的代表性例子进行了验证。
We report a label-free, structure-independent luminescent-sensing platform for metal ions, proteins, and small molecules utilizing an Ir(III) complex, terminal deoxynudeotidyl transferase (TdT), and a structure-folding aptamer. A novel G-quadruplex-selective Ir(III) complex was identified to detect the nascent G-quadruplex motifs with an enhanced luminescence response. Unlike most label-free DNA-based assays reported in the literature, this sensing platform does not require a specific secondary structure of aptamer, thus greatly simplifying DNA design. The detection platform was demonstrated by the detection of K+ ions, thrombin, and cocaine as representative examples of metal ions, proteins, and small molecules.