The effect of hydrostatic pressure on intervertebral disc metabolism
The effect of hydrostatic pressure on intervertebral disc metabolism
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DOI：
10.1097/00007632-199908010-00002
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发表时间：
1999-08-01
期刊：
影响因子：
3
通讯作者：
Hair, GA
中科院分区：
文献类型：
--
作者：
Hutton, WC;Elmer, WA;Hair, GA
研究设计.通过使用压力容器，对在藻酸盐中培养的椎间盘细胞施加静水压力。为了验证流体静压直接影响椎间盘细胞合成胶原蛋白和蛋白聚糖的假设。背景资料概要。压缩（流体静力学和机械）对软骨细胞代谢的影响在许多早期研究中进行了检查。然而，在大多数这些研究中，使用关节软骨，而不是椎间盘，并且在这些研究中，没有一个是静水压力施加到藻酸盐中培养的椎间盘细胞。从狗的腰椎间盘收获新鲜细胞。在藻酸盐凝胶系统中悬浮细胞之前，将细胞铺板并扩增直至它们达到汇合。然后，通过使用藻酸盐凝胶系统，将细胞暴露于两个不锈钢压力容器内特定值的流体静压下（长达9天）。一个容器保持在IMPa，另一个保持在大气压。通过测量[H-3]-脯氨酸和[S-35]-硫酸盐分别掺入到纤维环细胞和核细胞的胶原和蛋白多糖中，并通过确定这种掺入是否反映了聚集蛋白聚糖和I型和II型胶原的mRNA水平的变化，将1 MPa的作用与大气压进行比较。与大气压相比，发现：1）在掺入研究中，细胞核和纤维环细胞对1 MPa的静水压力表现出不同的反应。胶原和蛋白多糖的合成在核细胞中被刺激，在纤维环细胞中被抑制。2)在1 MPa下，无论是纤维环还是核细胞，细胞增殖都没有显著增加，这是通过DNA含量来测量的。3)在1 MPa压力下，核和纤维环细胞中胶原（Col 1A 1和Col 2A 1）和聚集蛋白聚糖的mRNA水平均升高。静水压直接影响椎间盘细胞合成胶原和蛋白多糖。
Study Design. By the use of pressure vessels, hydrostatic pressure was applied to intervertebral disc cells cultured in an alginate.Objective. To test the hypothesis that hydrostatic pressure directly affects the synthesis of collagen and proteo-glycan by the intervertebral disc cells.Summary of Background Data. The influence of compression (both hydrostatic and mechanical) on chondrocyte metabolism was examined in a number of earlier studies. However, in most of these studies, articular car tilage, not intervertebral disc, was used, and in none of these was hydrostatic pressure applied to intervertebral disc cells cultured in alginate.Methods. Fresh cells were harvested from the lumbar intervertebral discs of dogs. Before their suspension in an alginate gel system, the cells were plated and expanded until they reached confluence. Then, by use of the alginate gel system, the cells were exposed (for up to 9 days) to specific values of hydrostatic pressure inside two Stainless steel pressure vessels. One vessel was kept at 1 MPa and the other at atmospheric pressure. The effects of 1 MPa were compared against atmospheric pressure by measuring the incorporation of [H-3]-proline and [S-35]-sulfate into collagen and proteoglycans, respectively, for the anulus cells and nucleus cells separately, and by determining whether this incorporation was reflected by changes in the levels of mRNA for aggrecan and Types I and II collagen.Results. Comparisons with atmospheric pressure yielded the following findings: 1) In the incorporation studies, the nucleus and anulus cells exhibited a differential response to a hydrostatic pressure of 1 MPa. Collagen and proteoglycan syntheses were stimulated in the nucleus cells and inhibited in the anulus cells. 2) There was no significant increase in cell proliferation, as measured by DNA content, at 1 MPa for either the anulus or nucleus cells. 3) The mRNA levels of collagen (Col 1A1 and Col 2A1) and aggrecan increased at 1 MPa in both the nucleus and anulus cells.Conclusions. Hydrostatic pressure directly affects the synthesis of collagen and proteoglycan by the intervertebral disc cells.