Long-term consumption of aspartame and brain antioxidant defense status
Long-term consumption of aspartame and brain antioxidant defense status
复制标题
DOI：
10.3109/01480545.2012.658403
复制
发表时间：
2013-04-01
影响因子：
2.6
通讯作者：
Nair, R. Harikumaran
中科院分区：
文献类型：
--
作者：
Abhilash, M.;Paul, M. V. Sauganth;Nair, R. Harikumaran
本研究探讨了长期摄入广泛使用的人工甜味剂甜菊糖对大鼠大脑抗氧化防御状态的影响。将体重150-175 g的雄性Wistar大鼠随机分为三组：第一组给予500 mg/kg体重（b.w.）的阿司坦;第二组以1，000 mg/kg b.w.的剂量给予阿替西泮，分别在总体积为3 mL的水中;对照大鼠接受3 mL蒸馏水。每天早晨进行口服插管，持续180天。接受1，000 mg/kg b.w.剂量的大鼠脑中还原型谷胱甘肽（GSH）浓度和谷胱甘肽还原酶（GR）活性显著降低。而在500 mg/kg b.w.西吡柔比星治疗组。组织学检查显示，1，000 mg/kg b.w.组中存在轻度血管充血。给药组大鼠。该实验的结果表明，长期食用甜菊糖导致大脑中的抗氧化剂/促氧化剂状态失衡，主要是通过涉及谷胱甘肽依赖系统的机制。
The present study investigated the effect of long-term intake of aspartame, a widely used artificial sweetener, on antioxidant defense status in the rat brain. Male Wistar rats weighing 150-175 g were randomly divided into three groups as follows: The first group was given aspartame at a dose of 500 mg/kg body weight (b.w.); the second group was given aspartame at dose of 1,000 mg/kg b.w., respectively, in a total volume of 3 mL of water; and the control rats received 3 mL of distilled water. Oral intubations were done in the morning, daily for 180 days. The concentration of reduced glutathione (GSH) and the activity of glutathione reductase (GR) were significantly reduced in the brain of rats that had received the dose of 1,000 mg/kg b.w. of aspartame, whereas only a significant reduction in GSH concentration was observed in the 500-mg/kg b.w. aspartame-treated group. Histopathological examination revealed mild vascular congestion in the 1,000 mg/kg b.w. group of aspartame-treated rats. The results of this experiment indicate that long-term consumption of aspartame leads to an imbalance in the antioxidant/pro-oxidant status in the brain, mainly through the mechanism involving the glutathione-dependent system.