Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration
Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration
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DOI：
10.1007/978-1-4939-1714-3_43
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Wang, Lianchun
中科院分区：
文献类型：
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作者：
Qiu, Hong;Xiao, Wenyuan;Wang, Lianchun
硫酸乙酰肝素是一种具有硫酸化修饰的长链线性多糖，属于糖胺聚糖家族。我们最近的研究阐明，轴突导向分子Slit 3是一种新的硫酸乙酰肝素结合蛋白，并通过与其同源受体Robo 4相互作用而成为一种新的血管生成因子，Robo 4特异性地表达于内皮细胞中。在这里，我们描述了使用硫酸乙酰肝素缺陷的小鼠内皮细胞，以确定共接收功能的硫酸乙酰肝素在Slit 3诱导的内皮细胞迁移中的Boyden室trans-well迁移试验。
Heparan sulfate is a long, linear polysaccharide with sulfation modifications and belongs to the glycosaminoglycan family. Our recent studies elucidated that the axon guidance molecule Slit3 is a new heparan sulfate-binding protein and a novel angiogenic factor by interacting with its cognate receptor Robo4, which is specifically expressed in endothelial cells. Here we describe using heparan sulfate-deficient mouse endothelial cells to determine the co-reception function of heparan sulfate in Slit3-induced endothelial cell migration in a Boyden chamber trans-well migration assay.