Structure of the carbohydrate chains of the proline-rich glycoprotein from human parotid saliva.
Structure of the carbohydrate chains of the proline-rich glycoprotein from human parotid saliva.
复制标题
人腮腺唾液中富含脯氨酸的糖蛋白的碳水化合物链结构。
DOI：
10.1016/0006-291x(82)91331-6
复制
发表时间：
1982
影响因子：
3.1
通讯作者：
Tabak,LA
中科院分区：
文献类型：
--
作者：
Reddy,MS;Levine,MJ;Tabak,LA
本文报道了人腮腺唾液中富含脯氨酸的糖蛋白的结构。对通过半乳糖氧化酶/硼氚化钠处理的糖蛋白的广泛链霉蛋白酶消化制备的氚化糖肽进行了研究。使用高碘酸盐氧化、甲基化分析、亚硝酸脱氨基和糖苷酶处理的组合进行结构表征。发现一个三触角的天冬酰胺连接结构，其包含外周Galβ1→4（Fucα1→3）GlcNAc序列。
The carbohydrate structure of a proline-rich glycoprotein from human parotid saliva is presented. Studies were performed on tritiated glycopeptides prepared by extensive pronase digestion of galactose oxidase/sodium borotritide treated glycoprotein. Structural characterization was carried out using a combination of periodate oxidation, methylation analysis, nitrous acid deamination, and glycosidase treatment. A triantennary asparagine-linked structure was found which contained peripheral Galβ1→4 (Fucα1→3) GlcNAc sequences.