Generation of reelin-positive marginal zone cells from the caudomedial wall of telencephalic vesicles
Generation of reelin-positive marginal zone cells from the caudomedial wall of telencephalic vesicles
复制标题
DOI：
10.1523/jneurosci.4671-03.2004
复制
发表时间：
2004-03-03
影响因子：
5.3
通讯作者：
Tanabe, Y
中科院分区：
文献类型：
--
作者：
Takiguchi-Hayashi, K;Sekiguchi, M;Tanabe, Y
发育新皮质的层状组织的早期和基本步骤是由发育程序控制的，该程序主要取决于边缘区 reelin 阳性细胞的活动。然而，reelin 阳性细胞的个体发育仍然难以捉摸。为了深入了解 reelin 阳性边缘区细胞发育的空间和时间调控，我们使用了转基因小鼠品系，其中我们将绿色荧光蛋白 (GFP) 转基因定义为 reelin 阳性边缘区细胞发育早期阶段的新型可靠分子标记。我们进一步使用了子宫外电穿孔介导的基因转移，使我们能够标记祖细胞并监测体内端脑的后代。我们在这里展示了从端脑囊泡尾内侧壁（包括皮质下摆）产生的 reelin 阳性边缘区细胞，其中最初检测到 GFP 的显着表达。这些神经元在皮质边缘区切向迁移，并在皮质发生的动态发育时期以尾内侧高到头外侧低的梯度分布在整个新皮质中。因此，除了皮质中间神经元外，我们对 reelin 阳性边缘区细胞的发现补充了新皮质由神经元亚型组成的新观点，这些神经元亚型起源于新皮质外在的焦点源，切向迁移到新皮质中，从而成为新皮质神经组织的基础。
An early and fundamental step of the laminar organization of developing neocortex is controlled by the developmental programs that critically depend on the activities of reelin-positive cells in the marginal zone. However, the ontogeny of reelin-positive cells remained elusive. To gain insights into the spatial and temporal regulation of reelin-positive marginal zone cell development, we used a transgenic mouse line in which we defined the green fluorescent protein (GFP) transgene as a novel reliable molecular marker of reelin-positive marginal zone cells from the early stages of their development. We further used exo utero electroporation-mediated gene transfer that allows us to mark progenitor cells and monitor the descendants in the telencephalon in vivo. We show here the generation of reelin-positive marginal zone cells from the caudomedial wall of telencephalic vesicles, including the cortical hem, where the prominent expression of GFP is initially detected. These neurons tangentially migrate at the cortical marginal zone and are distributed throughout the entire neocortex in a caudomedial-high to rostrolateral-low gradient during the dynamic developmental period of corticogenesis. Therefore, our findings on reelin-positive marginal zone cells, in addition to the cortical interneurons, add to the emerging view that the neocortex consists of neuronal subtypes that originate from a focal source extrinsic to the neocortex, migrate tangentially into the neocortex, and thereby underlie neural organization of the neocortex.