ATRIAL NATRIURETIC FACTOR - A CIRCULATING HORMONE STIMULATED BY VOLUME LOADING
ATRIAL NATRIURETIC FACTOR - A CIRCULATING HORMONE STIMULATED BY VOLUME LOADING
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DOI：
10.1038/314264a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
UNGER, T
中科院分区：
文献类型：
--
作者：
LANG, RE;THOLKEN, H;UNGER, T
哺乳动物心房的心肌细胞含有与内分泌细胞非常相似的颗粒1,2。这些心房颗粒的数量与盐负荷和血容量直接相关。此外，大鼠心房粗提取物和颗粒制剂具有强大的利钠和利尿作用4,5。这些作用是由先前确定的心房钠素因子（ANF）肽介导的。这些肽来源于一个共同的前体，其结构最近已被阐明6 - 15。尽管形态学研究有间接证据表明，至少其中一些肽可能被释放到血液中并发挥激素的作用，但它们在血液中的存在尚未得到证实。在这里，我们描述了一种敏感和特异性的放射免疫测定法，用于ANF及其对体积负荷的刺激。
The cardiocytes of mammalian cardiac atria contain granules very similar to those in endocrine cells1,2. The number of these atrial granules is related directly to salt loading and blood volume3. Furthermore, crude extracts of rat atria and granule preparations have powerful natriuretic and diuretic effects4,5. These effects are mediated by peptides identified previously as atrial natriuretic factor (ANF). The peptides are derived from a common precursor, whose structure has been elucidated recently6–15. Although there is indirect evidence from morphological studies that at least some of these peptides may be released into the blood and function as hormones, their presence in the blood has not yet been demonstrated. Here we describe a sensitive and specific radio-immunoassay for ANF and its stimulation on volume loading.