High oxygen radical production ts associated with fast parasite clearance in children with Plasmodium falciparum malaria
High oxygen radical production ts associated with fast parasite clearance in children with Plasmodium falciparum malaria
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DOI：
10.1086/314780
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发表时间：
1999-06-01
影响因子：
6.4
通讯作者：
Kremsner, PG
中科院分区：
文献类型：
--
作者：
Greve, B;Lehman, LG;Kremsner, PG
据推测，白细胞释放的活性氧中间体（ROI）在对许多感染因子的免疫应答中起主要作用。在本研究中，75名加蓬儿童恶性疟原虫疟疾的寄生虫学和临床过程进行了比较，其粒细胞产生氧自由基的能力。儿童粒细胞悬液中的鲁米诺依赖性化学发光在无刺激和用佛波醇-12-肉豆蔻酸酯-13-乙酸酯、N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸或肿瘤坏死因子刺激后测定。在未受刺激和受刺激的粒细胞制剂中，发现快速寄生虫清除时间和高氧自由基生成之间存在显著关联。未发现发烧消退时间与感兴趣区产生之间存在相关性。这些发现表明，ROI在免疫应答中起着关键作用，是对抗恶性疟原虫疟疾的第一道防线。
It has been hypothesized that reactive oxygen intermediates (ROI) released by leukocytes play a major role in the immune response to many infectious agents. Tn the present study, the parasitologic and clinical courses of 75 Gabonese children with Plasmodium falciparum malaria were compared with the ability of their granulocytes to produce oxygen radicals. The luminol-dependent chemiluminescence in granulocyte suspensions for the children was measured without stimulation and after stimulation with phorbol-12-myristate-13-acetate, N-formyl-methionyl-leucyl-phenylalanine, or tumor necrosis factor. A significant association was found between fast parasite clearance time and high oxygen radical generation in both the unstimulated and stimulated granulocyte preparations. No correlation was found between fever clearance time and ROI generation. These findings suggest that ROI play a pivotal role in the immune response as a first line of defense against P. falciparum malaria.