Imaging human epithelial properties with polarized light-scattering spectroscopy
Imaging human epithelial properties with polarized light-scattering spectroscopy
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DOI：
10.1038/nm1101-1245
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发表时间：
2001-11-01
期刊：
影响因子：
82.9
通讯作者：
Feld, MS
中科院分区：
文献类型：
--
作者：
Gurjar, RS;Backman, V;Feld, MS
光散射光谱生物医学成像是一种新的光学技术，用于原位探测活体上皮细胞的结构，而无需去除组织。LSS使得区分上皮细胞核的单次后向散射和多次散射光成为可能。进一步分析单个反向散射分量的光谱以提供关于上皮细胞核的定量信息，例如核大小、多形性程度、染色质增多程度和染色质量。LSS成像允许将这些组织学特性映射到上皮衬里的广泛区域。由于核增大、多形性和染色质增多是几乎所有上皮细胞中与癌前病变和癌性变化相关的核异常的主要特征，因此LSS成像可用于检测光学可及器官中的癌前病变。
Biomedical imaging with light-scattering spectroscopy (LSS) is a novel optical technology developed to probe the structure of living epithelial cells in situ without need for tissue removal. LSS makes it possible to distinguish between single backscattering from epithelial-cell nuclei and multiply scattered light. The spectrum of the single backscattering component is further analyzed to provide quantitative information about the epithelial-cell nuclei such as nuclear size, degree of pleomorphism, degree of hyperchromasia and amount of chromatin. LSS imaging allows mapping these histological properties over wide areas of epithelial lining. Because nuclear enlargement, pleomorphism and hyperchromasia are principal features of nuclear atypia associated with precancerous and cancerous changes in virtually all epithelia, LSS imaging can be used to detect precancerous lesions in optically accessible organs.