METABOLITES OF ERWINIA .9. PROFILING OF BASIC-AMINO-ACIDS AND POLYAMINES IN MICROBIAL CULTURE SUPERNATANTS BY ELECTROSPRAY MASS-SPECTROMETRY
METABOLITES OF ERWINIA .9. PROFILING OF BASIC-AMINO-ACIDS AND POLYAMINES IN MICROBIAL CULTURE SUPERNATANTS BY ELECTROSPRAY MASS-SPECTROMETRY
复制标题
DOI：
10.1002/bms.1200231211
复制
发表时间：
1994-12-01
期刊：
影响因子：
--
通讯作者：
FEISTNER, GJ
中科院分区：
文献类型：
--
作者：
FEISTNER, GJ
As part of our efforts to investigate the biosynthesis of proferrioxamines in Erwinia amylovora, it was of interest to develop a methodology with which a large number of basic amino acids, di- and polyamines, and C- and N-hydroxy derivatives thereof could be monitored simultaneously. Towards this end, the on-line coupling of electrospray mass spectrometry with ion chromatography or reversed-phase chromatography was explored, Tandem mass spectrometry was found to be an excellent method for obtaining relevant structural information on underivatized polyamines and basic amino acid, including N-hydroxy derivatives. However, the coupling of ion chromatography with electrospray mass spectrometry for on-line extraction and separation of the underivatized compounds is hampered by compatibility problems. For most target compounds, on-line reversed-phase liquid chromatography/electrospray mass spectrometry was the method of choice but required derivatization and in this respect dansylation was preferred over benzoylation. Neither derivatization method is suitable for N-hydroxy compounds, which are base-sensitive.
