Immunoregulatory effect of human beta-defensin 1 on neonatal cord blood monocyte-derived dendritic cells and T cells
Immunoregulatory effect of human beta-defensin 1 on neonatal cord blood monocyte-derived dendritic cells and T cells
复制标题
人β-防御素1对新生儿脐血单核细胞衍生树突状细胞和T细胞的免疫调节作用
DOI：
10.1016/j.molimm.2019.03.007
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发表时间：
2019
影响因子：
3.6
通讯作者：
Chen Tong Xin
中科院分区：
文献类型：
--
作者：
Wu Jing;Gong Ruo Lan;Hu Qing Feng;Chen Xu Ting;Zhao Wei;Chen Tong Xin
母乳喂养和婴儿健康之间的关系在过去几十年中得到了很好的阐述。我们前期的研究表明，母乳中的人β-防御素1（hBD-1）对降低6个月以下婴儿上呼吸道感染的发生率具有保护作用。在本研究中，我们的目的是揭示hBD-1的保护作用的机制，通过关注其在新生儿的免疫调节功能。来自新生儿脐带的脐带血（CB）可以模拟许多新生儿症状，结果表明，hBD-1可促进GM-CSF和IL-4诱导的新生脐血单核细胞向未成熟树突状细胞（DC）分化，并促进CB单核细胞衍生的DC（moDC）的最终成熟LPS诱导而非炎性细胞因子产生。此外，hBD-1通过CCR 6抑制新生moDC的凋亡，这可能是hBD-1诱导moDC表型改变的一个可能机制。此外，我们发现hBD-1促进新生儿CB CD 4 + T细胞的增殖和活化，但不促进其成熟。这些结果扩展了hBD-1的免疫调节作用，并为hBD-1在早期婴儿中的保护作用提供了潜在的机制，这将为未来婴儿营养，新型疫苗和抗感染策略的开发提供信息。
The relationship between breastfeeding and infant health has been well elucidated in past decades. Our previous study has shown that human β-defensin 1 (hBD-1) in human breast milk plays a protective role in reducing the incidence of upper respiratory infection in infants younger than 6 months. In the present study, we aim to reveal the mechanism underlying the protective role of hBD-1 by focusing on its immunoregulatory function in neonates. Cord blood (CB) from newborns’ umbilical cords, which can simulate many of the neonatal symptoms, was used to study the immunomodulatory role of hBD-1 in neonatesin vitro.Our results showed that hBD-1 promotes the GM-CSF- and IL-4-driven differentiation of neonatal umbilical CB monocytes to immature dendritic cells (DCs) and the final maturation of CB monocyte-derived DCs (moDCs) induced by LPS but not inflammatory cytokine production. In addition, hBD-1 inhibits apoptosis in neonatal moDCs through CCR6, which might be a possible mechanism of the hBD-1-induced phenotypes in moDCs. Furthermore, we found that hBD-1 promotes the proliferation and activation, but not the maturation, of neonatal CB CD4 + T cells. These results extend the immunoregulatory effects of hBD-1 and provide a potential mechanism for the protective role of hBD-1 in early infants, which will inform the development of infant nutrition, novel vaccines and anti-infective strategies in the future.