Preparation and evaluation of heparin-immobilized poly (lactic acid) (PLA) membrane for hemodialysis
Preparation and evaluation of heparin-immobilized poly (lactic acid) (PLA) membrane for hemodialysis
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DOI：
10.1016/j.memsci.2013.10.016
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发表时间：
2014-02-15
影响因子：
9.5
通讯作者：
Xue, Lixin
中科院分区：
文献类型：
--
作者：
Gao, Ailin;Liu, Fu;Xue, Lixin
首次开发出具有不对称多孔结构的生物基聚乳酸膜，用于通过相转化进行血液透析。衰减全反射傅里叶变换红外（ATR-FTIR）光谱和X射线光电子能谱（XPS）分别证实了肝素通过多巴胺的强粘附能力固定在PLA膜表面。通过扫描电子显微镜（SEM）和原子力显微镜（AFM）分析了多巴胺涂层和肝素固定引起的PLA膜的形貌变化。对改性前后PLA膜的亲水性和渗透性进行了表征。特别是通过血小板吸附、血浆再钙时间和溶血率来评价肝素修饰的PLA膜的血液相容性。体外结果表明，表面肝素化可改善PLA膜的血液相容性，抑制血小板粘附，延长血浆重钙时间，降低溶血率。进行尿素和溶菌酶清除以及牛血清白蛋白（BSA）排斥的透析模拟实验来确定透析性能。结果显示，79%的尿素和18%的溶菌酶被净化，90%以上的BSA被保留。这项研究揭示了使用生物基材料生产新型血液透析膜的机会之窗。 (C) 2013 Elsevier B.V. 版权所有
Bio-based poly (lactic acid) membrane with asymmetric porous structure was developed for hemodialysis via phase inversion for the first time. Heparin was immobilized to PLA membrane surface through the strong adhesion ability of dopamine, as confirmed by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) respectively. The morphologies variations of PLA membranes induced by dopamine coating and heparin immobilization were analyzed by scanning electron microscope (SEM) and atomic force microscopy (AFM). Hydrophilicity and permeability of PLA membranes before and after modification were characterized. Particularly, platelet adsorption, plasma recalcification time and hemolysis ratio were executed to evaluate the blood compatibility of PLA membranes decorated by heparin. The in vitro results demonstrated that surface heparinization improved the hemocompatibility of PLA membrane, suppressed the adhesion of platelet, extended plasma recalcilication time, and also decreased hemolysis ratio. The dialysis simulation experiments including urea and lysozyme clearance as well as bovine serum albumin (BSA) rejection were implemented to determine the dialysis performances. The results showed that 79% of urea and 18% of lysozyme were cleaned and over 90% of BSA was retained. This study disclosed a window of opportunity to produce novel hemodialysis membranes using bio-based materials. (C) 2013 Elsevier B.V. All rights reserved