Peroxidative degradation of selected PCB:: a mechanistic study
Peroxidative degradation of selected PCB:: a mechanistic study
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DOI：
10.1016/s0045-6535(00)00132-6
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发表时间：
2000-12-01
期刊：
影响因子：
8.8
通讯作者：
Czihal, K
中科院分区：
文献类型：
--
作者：
Köller, G;Möder, M;Czihal, K
研究了多氯联苯的酶促分解和生物降解。以2,5-二氯联苯（PCB 9）和2,2',5,5'-四氯联苯（PCB 52）为例，研究了降解过程的效率和机理。结果发现，在反应220分钟后，将辣根过氧化物酶（HRP）与一定量的过氧化氢一起从水溶液中去除初始浓度的PCB 9的90%和初始浓度的PCB 52的55%。脱氯是观察到的第一步。虽然鉴定出的代谢物主要是氯代羟基联苯、苯甲酸和非取代的1,1'-联苯，但也出现了一些更高氯代联苯异构体。多色白腐菌对PCB 9的生物降解时间约为4周，降解浓度约为初始浓度的80%。产生的代谢物（二氯苯、氯酚和烷基化苯）与HRP孵育时观察到的不完全相同。(C) 2000 Elsevier Science Ltd.版权所有。
The enzyme-induced decomposition and biodegradation of PCB were investigated. 2,5-Dichlorobiphenyl (PCB 9) and 2,2',5,5'-tetrachlorobiphenyl (PCB 52) were used as example compounds to study efficiency and mechanism of the degradation processes. It was found that the application of horseradish peroxidase (HRP) together with defined amounts of hydrogen peroxide removed 90% of the initial concentration of PCB 9 and 55% of the initial concentration of PCB 52 from an aqueous solution after a reaction period of 220 min. Dechlorination was observed as the initial step. Although the metabolites identified were mainly chlorinated hydroxybiphenyls, benzoic acids and non-substituted 1,1'-biphenyl, some higher chlorinated biphenyl isomers also appeared. The biodegradation of PCB 9 using the white rot fungus Trametes multicolor took about four weeks and reduction was about 80% of the initial concentration. The metabolites produced (dichlorobenzenes, chlorophenols and alkylated benzenes) were not quite the same as those observed upon incubation with HRP. (C) 2000 Elsevier Science Ltd. All rights reserved.