Assessing DNA Damage Responses Using B Lymphocyte Cultures.
Assessing DNA Damage Responses Using B Lymphocyte Cultures.
复制标题
使用 B 淋巴细胞培养物评估 DNA 损伤反应。
DOI：
10.1007/978-1-0716-2063-2_5
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Bednarski,JeffreyJ
中科院分区：
文献类型：
--
作者：
Johnston,Rachel;White,LynnS;Bednarski,JeffreyJ
B细胞的发育需要抗原受体基因中双链DNA断裂的程序性生成和修复。对这些DNA断裂的细胞反应的研究已经建立了对B细胞发育的重要见解，更广泛地说，为DNA损伤反应途径的分子机制提供了基本的进展。Abelson转化的Pre-B细胞系和原代Pre-B细胞培养是具有延展性的实验系统，在研究DNA损伤反应方面有不同的应用。本章描述了生成这些细胞系统、诱导和量化DSB以及评估DNA损伤程序的方法。
Development of B cells requires the programmed generation and repair of double-stranded DNA breaks in antigen receptor genes. Investigation of the cellular responses to these DNA breaks has established important insights into B cell development and, more broadly, has provided fundamental advances into the molecular mechanisms of DNA damage response pathways. Abelson transformed pre-B cell lines and primary pre-B cell cultures are malleable experimental systems with diverse applications for studying DNA damage responses. This chapter describes methods for generating these cellular systems, inducing and quantifying DSBs, and assessing DNA damage programs.