Cultured capillary endothelial cells from bovine adipose tissue: a model for insulin binding and action in microvascular endothelium.
Cultured capillary endothelial cells from bovine adipose tissue: a model for insulin binding and action in microvascular endothelium.
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来自牛脂肪组织的培养毛细血管内皮细胞：微血管内皮中胰岛素结合和作用的模型。
DOI：
10.1016/0026-0495(86)90147-2
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Boes,M
中科院分区：
文献类型：
--
作者：
Bar,RS;Dolash,S;Dake,BL;Boes,M
从牛脂肪组织培养毛细血管内皮细胞。通过特征形态学、因子VIII抗原的均匀存在和内皮细胞标记物Dil-Ac-LDL的摄取记录培养物的内皮性质。毛细血管细胞培养物对胰岛素具有特异性、高亲和力结合位点，证明了结合的时间和温度依赖性、最佳pH值、类似物特异性和抗胰岛素受体抗体对胰岛素结合的抑制。在亚汇合和汇合培养物中，胰岛素刺激胸苷掺入DNA;在胰岛素浓度为1 ng/ml时观察到胰岛素的显著刺激作用，在激素浓度为1，000至10，000 ng/ml时最大增加8至10倍。由于易于常规制备，细胞纯度，高亲和力的胰岛素结合位点的存在下，和胰岛素敏感的代谢反应，我们建议，牛毛细血管内皮细胞培养可以作为一个模型细胞系统的胰岛素与毛细血管内皮细胞的相互作用的详细研究。
Capillary endothelial cells were cultured from bovine adipose tissue. The endothelial nature of the cultures was documented by characteristic morphology, uniform presence of factor VIII antigen, and uptake of the endothelial cell marker Dil-Ac-LDL. The capillary cell cultures had specific, high affinity binding sites for insulin, demonstrating time and temperature dependence of binding, pH optimum, analog specificity, and inhibition of insulin binding by anti-insulin receptor antibodies. In both subconfluent and confluent cultures, insulin stimulated thymidine incorporation into DNA; significant stimulatory effects of insulin were observed at insulin concentrations of 1 ng/ml with maximal 8- to 10-fold increases at hormone concentrations of 1,000 to 10,000 ng/ml. Because of the ease of routine preparation, cell purity, presence of high affinity insulin binding sites, and insulin-sensitive metabolic responses, we suggest that the bovine capillary endothelial cultures could serve as a model cell system for the detailed study of insulin interactions with capillary endothelial cells.