Circular RNA expression profiling of granulosa cells in women of reproductive age with polycystic ovary syndrome
Circular RNA expression profiling of granulosa cells in women of reproductive age with polycystic ovary syndrome
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多囊卵巢综合征育龄妇女颗粒细胞的环状 RNA 表达谱
DOI：
10.1007/s00404-019-05129-5
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发表时间：
2019-04
影响因子：
2.6
通讯作者：
Ma Hongxia
中科院分区：
文献类型：
--
作者：
Zhang Chunren;Liu Jianqiao;Lai Maohua;Li Juan;Zhan Jiahui;Wen Qidan;Ma Hongxia
目的探讨多囊卵巢综合征(PCOS)育龄妇女颗粒细胞CircRNA的表达情况。方法从15例PCOS患者和15例体重指数、年龄匹配的健康妇女卵巢颗粒细胞中提取总RNA。对去除核糖体的RNA进行RNA测序，以获得CircRNA表达谱。通过分级聚集热图和火山图，比较和可视化了多囊卵巢综合征患者和对照组之间CircRNA的差异表达。基因本体论(GO)和京都百科全书的基因和基因组路径丰富分析确定了CircRNAs差异表达的作用。结果根据RNA测序数据，PCOS和对照组分别预测了4258和7395个候选CircRNAs。两组CircRNA的表达模式存在差异。表达谱分析显示，在多囊卵巢综合征患者中，4个CircRNA上调，23个下调。GO分析表明，这27个差异表达的CircRNA主要分布在生物过程中，特别是在炎症、增殖和血管内皮生长因子相关的信号通路中。使用不同的引物在多囊卵巢综合征患者中鉴定出六个CircRNA。结论多囊卵巢综合征患者多囊卵巢组织中存在多个环状RNA的表达，提示这些环状RNA参与了多囊卵巢综合征的发生发展过程及其作为多囊卵巢综合征生物标志物的临床意义。
PurposeThis study aimed to explore the expression profiles of circRNA in granulosa cells of women of reproductive age with polycystic ovary syndrome (PCOS).MethodsTotal RNA was isolated from granulosa cells of 15 women with PCOS and 15 body mass index- and age-matched healthy women (control). RNA sequencing was conducted on ribosomal-depleted RNA for circRNA expression profiling. The differential expression of circRNA between women with PCOS and controls was compared and visualized using hierarchical clustering heat maps and Volcano plots. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed to determine the role of the differential expression of circRNAs. The expression rates of circRNAs were confirmed using quantitative real-time PCR (qRT-PCR) using divergent primers.ResultsA total of 4258 and 7395 candidate circRNAs were predicted in PCOS and controls, respectively, based on the RNA-sequencing data. Differences were noted in the expression patterns of circRNA between the two groups. Analysis of the expression profiles revealed that four circRNAs were upregulated, whereas 23 were downregulated in the women with PCOS. GO analysis suggested that the 27 differentially expressed circRNAs were mainly distributed in biological process pathways, particularly in pathways involving inflammation, proliferation, and the vascular endothelial growth factor-related signaling pathway. Six circRNAs were identified in PCOS-affected women using divergent primers. qRT-PCR confirmed that hsa_circ_0001577 was significantly upregulated and hsa_circ_0020093 was downregulated in the women with PCOS.ConclusionsSeveral circRNAs were differentially expressed in women of reproductive age with PCOS, suggesting the involvement of these circRNAs in the development of PCOS and the potential clinical implications of their use as PCOS biomarkers.
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影响因子：
1
作者：
Aghadavod E;Zarghami N;Farzadi L;Zare M;Barzegari A;Movassaghpour AA;Nouri M
通讯作者：
Nouri M
DOI：
10.1056/nejmcp1514916
发表时间：
2016-07-07
期刊：
The New England journal of medicine
影响因子：
--
作者：
McCartney CR;Marshall JC
通讯作者：
Marshall JC
DOI：
10.1056/nejm200506303522620
发表时间：
2005-06
期刊：
The New England journal of medicine
影响因子：
--
作者：
L. Mascitelli;F. Pezzetta
通讯作者：
L. Mascitelli;F. Pezzetta
影响因子：
4
作者：
Kanamarlapudi, Venkateswarlu;Gordon, Uma D.;Bernal, Andres Lopez
通讯作者：
Bernal, Andres Lopez
DOI：
10.1201/9780203910948
发表时间：
1992-02
期刊：
Current therapy in endocrinology and metabolism
影响因子：
--
作者：
R. Azziz;E. Carmina;Zi-jiang Chen;A. Dunaif;J. Laven;R. Legro;Daria Lizneva;Barbara Natterson-Horowtiz-Barbara-Natte
通讯作者：
R. Azziz;E. Carmina;Zi-jiang Chen;A. Dunaif;J. Laven;R. Legro;Daria Lizneva;Barbara Natterson-Horowtiz-Barbara-Natte