Cytochrome oxidase from thermophilic bacterium PS3 contains a fourth protein subunit.
Cytochrome oxidase from thermophilic bacterium PS3 contains a fourth protein subunit.
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来自嗜热细菌 PS3 的细胞色素氧化酶含有第四个蛋白质亚基。
DOI：
10.1016/0006-291x(90)90347-p
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发表时间：
1990
影响因子：
3.1
通讯作者：
Chan,SH
中科院分区：
文献类型：
--
作者：
Gai,WZ;Sun,SM;Sone,N;Chan,SH
发现针对牛心细胞色素氧化酶亚基 II 和 IV 制备的单克隆抗体与嗜热细菌 PS3 氧化酶发生交叉反应。每个单独的抗体都会影响酶活性。 “Western”印迹分析表明，牛心的亚基 II 抗体识别 PS3 的亚基 II，而亚基 IV 抗体同样识别平板凝胶上的第四个蛋白质亚基。该第四个亚基先前被认为是污染物或降解产物，在 SDS 凝胶上的分子量约为 10,500，并且似乎以化学计量存在。我们从平板凝胶中提取了该亚基，并将其氨基酸组成与亚基 III 的氨基酸组成进行了比较。
Monoclonal antibodies prepared against subunits II and IV of beef heart cytochrome oxidase were found to cross-react with thermophilic bacterial PS3 oxidase. Each individual antibody affects the enzymatic activity. “Western” blot analyses showed that subunit II antibodies of beef heart recognized subunit II of PS3 and subunit IV antibody likewise recognized a fourth protein subunit on slab gels. This fourth subunit previously thought to be a contaminant or a degradation product has a molecular weight of about 10,500 on SDS-gels, and appears to exist in stoichiometric amount. We have extracted this subunit from slab gels and compared its amino acid composition with that of subunit III.