Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts.
Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts.
复制标题
苯妥英对人牙龈成纤维细胞产生白细胞介素 6 和白细胞介素 8 的影响。
DOI：
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发表时间：
2000
影响因子：
3.3
通讯作者：
G. Brunius
中科院分区：
文献类型：
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作者：
T. Modéer;H. Domeij;I. Andurén;M. Mustafa;G. Brunius
研究了苯妥英 (PHT) 对人牙龈成纤维细胞产生白细胞介素 6 (IL-6) 和白细胞介素 8 (IL-8) 的体外影响，无论是否有白细胞介素 1β (IL-1β) 攻击。在用 IL-1beta (30 pg/ml) 攻击的成纤维细胞中，单独使用 PHT (20 µg/ml) 可以增加 IL-6 和 IL-8 的 mRNA 水平，并在转录和翻译水平上协同增强 IL-6 和 IL-8 的产生。 PHT 对 IL-1β 诱导的 IL-6 产生的刺激作用被特定的环氧合酶 2 抑制剂 NS-398 (1 microM) 强烈降低。在存在或不存在 IL-1β 的情况下，用 PHT 攻击的牙龈成纤维细胞中，抗炎药物地塞米松 (1 µM) 消除了 IL-6 和 IL-8 的产生。 PHT 单独以及与 IL-1 组合，上调人牙龈成纤维细胞中 IL-6 和 IL-8 产生的能力可能有助于增强炎症细胞的募集和激活。因此，PHT 的这种作用可能为牙周组织中细胞因子和结缔组织细胞之间建立相互作用提供了先决条件，这被认为会导致牙龈过度生长。
The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1beta (IL-1beta), was studied. PHT (20 microg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1beta (30 pg/ml). The stimulatory effect of PHT on IL-1beta-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 microM). The anti-inflammatory drug, dexamethasone (1 microM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1beta. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth.