Analysis of QTLs for yield-related traits in Yuanjiang common wild rice (Oryza rufipogon Griff.)
Analysis of QTLs for yield-related traits in Yuanjiang common wild rice (Oryza rufipogon Griff.)
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DOI：
10.1016/s1673-8527(09)60033-3
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发表时间：
2010-02-01
影响因子：
5.9
通讯作者：
Sun, Chuanqing
中科院分区：
文献类型：
--
作者：
Fu, Qiang;Zhang, Peijiang;Sun, Chuanqing
利用普通野生稻（Oryza rujipogon Griff.）以云南元江的93-11为供体，以我国籼型两系杂交稻生产中广泛使用的优良品种93-11为轮回亲本，构建了所有的高代回交群体。通过对187个SSR标记的基因分型和对BC（4）F（2）和BC（4）F（4）两个世代6个产量相关性状的调查，采用单点分析和区间作图法在两个世代共检测到26个QTL。在26个QTL中，有10个（38.5%）来自O.在93-11遗传背景下，普通草对产量相关性状表现出有益的影响。此外，新发现了5个控制产量及其构成因素的QTL，表明元江普通野生稻中存在潜在的新等位基因。在第1、7和12染色体上检测到3个与产量相关的QTL区域。结果表明，这些QTL聚类后，其对应的农艺性状间存在极显著的相关性，表明QTL间存在多效性或紧密连锁。对这些野生稻产量相关QTL进行精细定位和克隆，将有助于进一步阐明水稻驯化和育种的分子机理。
Using an accession of common wild rice (Oryza rujipogon Griff.) collected from Yuanjiang County, Yunnan Province, China, as the donor and an elite cultivar 93-11, widely used in two-line indica hybrid rice production in China, as the recurrent parent, ail advanced backcross populations were developed. Through genotyping of 187 SSR markers and investigation of six yield-related traits of two generations (BC(4)F(2) and BC(4)F(4)), a total of 26 QTLs were detected by employing single point analysis and interval mapping in both generations. Of the 26 QTLs, the alleles of 10 (38.5%) QTLs originating from O. rufipogon had shown a beneficial effect for yield-related traits in the 93-11 genetic background. In addition, five QTLs controlling yield and its components were newly identified, indicating that there are potentially novel alleles in Yuanjiang common wild rice. Three regions underling significant QTLs for several yield-related traits were detected on chromosome 1, 7 and 12. The QTL clusters were founded and corresponding agronomic traits of those QTLs showed highly significant correlation, suggesting the pleiotropism or tight linkage. Fine-mapping and cloning of these yield-related QTLs from wild rice would be helpful to elucidating molecular mechanism of rice domestication and rice breeding in the future.