"Microcanals" for micropipette access to single cells in microfluidic environments
"Microcanals" for micropipette access to single cells in microfluidic environments
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DOI：
10.1039/b404956j
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发表时间：
2004-01-01
期刊：
影响因子：
6.1
通讯作者：
Folch, A
中科院分区：
文献类型：
--
作者：
Hsu, CH;Chen, CC;Folch, A
我们演示了“微管”的制造和操作（即没有屋顶的露天微流控通道），这使得微移液管可以在微流控环境中操作和探测细胞。采用PDMS膜转移技术在玻璃基板上的PDMS中制备微管器件。在这里，我们展示了膜片钳电生理记录和细胞内染料注射对微管细胞的植入。
We demonstrate the fabrication and operation of "microcanals'' (i.e. open-air microfluidic channels without a roof), which enable micropipette manipulation and probing of cells within a microfluidic environment. The microcanal devices are fabricated in PDMS on glass substrates using a PDMS membrane transferring technique. Here we show patch-clamp electrophysiological recording and intracellular dye injection performed on cells seeded in microcanals.