Nuclear factor 1 X-type-associated regulation of myogenesis in developing mouse tongue
Nuclear factor 1 X-type-associated regulation of myogenesis in developing mouse tongue
复制标题
核因子 1 X 型相关的小鼠舌头肌生成调节
DOI：
10.1016/j.job.2023.01.003
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Soeno Y
中科院分区：
文献类型：
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作者：
Kawamoto S;Hani T;Fujita K;Taya Y;Sasaki Y;Kudo T;Sato K;Soeno Y
目的舌中含有不同于躯干、四肢和其他口面部肌肉的骨骼肌纤维。然而，舌肌成肌分化的分子基础仍不清楚。在本研究中，我们对发育中的小鼠舌头进行了全面的基因表达谱分析。方法从胚胎第10.5-E18天的小鼠胚胎中解剖同种原基。5、通过基因芯片分析和定量聚合酶链式反应(PCR)检测肌源性标志物。除了常见的成肌调节因子Myf5、MyoD、Mygenin和MRf4外，我们还重点研究了Nfix，它是一种独特的分子开关，在肢体肌肉发生过程中触发了从胚胎成肌细胞向胎儿成肌细胞谱系的转变。结果基因芯片和独创性通路分析证实，在胚胎10天，成肌细胞和成肌祖细胞分化过程中，包括NFIX在内的成肌信号分子显著上调。5-E11。5.定量聚合酶链式反应证实Nfix基因在胚胎10天开始表达。5，在E14达到高峰。5.胎儿成肌细胞特异性基因，如Mck和Myh8，在胚胎14d后上调。5，而胚胎成肌细胞特异性基因，如Myh3和Myh7，表达下调。当Nfix在舌原基的器官培养中被抑制时，舌体内观察到细微的形态差异。这样的观察只在E10的文化中被注意到。结论Nfix在舌肌发生中起重要作用。NFIX在舌发育早期的表达可能在舌肌发育中起重要作用。
ObjectivesThe tongue contains skeletal myofibers that differ from those in the trunk, limbs, and other orofacial muscles. However, the molecular basis of myogenic differentiation in the tongue muscles remains unclear. In this study, we conducted comprehensive gene expression profiling of the developing murine tongue.MethodsTongue primordia were dissected from mouse embryos at embryonic day (E) 10.5–E18. 5, while myogenic markers were detected via microarray analysis and quantitative polymerase chain reaction (PCR). In addition to common myogenic regulatory factors such as Myf5, MyoD, myogenin, and Mrf4, we focused on Nfix, which acts as a unique molecular switch triggering the shift from embryonic to fetal myoblast lineage during limb myogenesis. Nfix inhibition was performed using a specific antisense oligonucleotide in the organ culture of tongue primordia.ResultsMicroarray and ingenuity pathway analyses confirmed the significant upregulation of myogenic signaling molecules, including Nfix, associated with the differentiation of myoblasts from myogenic progenitor cells during E10. 5–E11. 5. Quantitative PCR confirmed that Nfix expression started at E10. 5 and peaked at E14. 5. Fetal myoblast-specific genes, such as Mck and Myh8, were upregulated after E14. 5, whereas embryonic myoblast-specific genes, such as Myh3 and Myh7, were downregulated. When Nfix was inhibited in the organ culture of tongue primordia, subtle morphological differences were noted in the tongue. Such an observation was only noted in the cultures of E10. 5-derived tongue primordia.ConclusionsThese results reveal the contribution of Nfix to tongue myogenesis. Nfix expression during early tongue development may play a vital role in tongue muscle development.