Gibberellin A(1) Biosynthesis in Pisum sativum L. : II. Biological and Biochemical Consequences of the le Mutation.
Gibberellin A(1) Biosynthesis in Pisum sativum L. : II. Biological and Biochemical Consequences of the le Mutation.
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豌豆中赤霉素 A(1) 的生物合成：II。
DOI：
10.1104/pp.99.2.372
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发表时间：
1992
期刊：
影响因子：
7.4
通讯作者：
V. Smith
中科院分区：
文献类型：
--
作者：
V. Smith
本文报道了放射性标记赤霉素（GA） 1、19和20在等基因豌豆和豌豆（Pisum sativum）离体培养的离体营养组织中代谢的比较研究。本研究结果证明，参与GA生物合成后期阶段的酶在生长中的豌豆植物中是空间分离的。根尖芽不明显参与生物活性GA(1)或其直接前体的产生。GA（19）合成GA（20）的主要位点是未成熟的小叶和卷须，具有生物活性的GA(1)及其无活性分解产物GA(8)的合成主要发生在茎组织中。GA(29)， GA（20）的无活性分解代谢物，在所有被检查的组织中都不同程度地产生。相应的Le和Le组织代谢放射性标记的GA(1)、GA（19）甚至GA（20）的能力几乎没有差异。在固定的24小时内，携带le突变的植株茎部产生的[(3)H]GA(1)(和[（3)H]GA(29)）略高于le植株。已经得出结论，le突变不在编码GA(20) 3 β -羟化酶蛋白的基因内。
A comparative study of the metabolism of radiolabeled gibberellin (GA) 1, 19, and 20 in isolated vegetative tissues of isogenic Le and le pea (Pisum sativum) plants incubated in vitro with the appropriate GA substrate is described. The results of this study provide evidence that the enzymes involved in the latter stages of GA biosynthesis are spatially separated within the growing pea plant. Apical buds were not apparently involved in the production of bioactive GA(1) or its immediate precursors. The primary site of synthesis of GA(20) from GA(19) was immature leaflets and tendrils, and the synthesis of bioactive GA(1) and its inactive catabolite GA(8) occurred predominantly in stem tissue. GA(29), the inactive catabolite of GA(20), was produced to varying extents in all the tissues examined. Little or no difference was observed in the ability of corresponding Le and le tissues to metabolize radiolabeled GA(1), GA(19), or even GA(20). During a fixed period of 24 hours, stems of plants carrying the le mutation produced slightly more [(3)H]GA(1) (and [(3)H]GA(29)) than those of Le plants. It has been concluded that the le mutation does not lie within the gene encoding the GA(20) 3beta-hydroxylase protein.