Tissue resistance to insulin stimulation of amino acid uptake in acutely uremic rats.
Tissue resistance to insulin stimulation of amino acid uptake in acutely uremic rats.
复制标题
急性尿毒症大鼠对胰岛素刺激氨基酸摄取的组织抵抗。
作者：
W. Arnold;M. Holliday
外周组织抵抗胰岛素介导的葡萄糖摄取发生在尿毒症中。对胰岛素介导的氨基酸摄取也可能存在类似的抗性。本研究考察了胰岛素对双侧肾切除急性尿毒症大鼠组织中α -氨基异丁酸（AIBA）摄取或分布比（DR）的影响。在没有胰岛素的情况下，尿毒症大鼠和对照组大鼠在腓肠肌中的AIBA浓度相同（对照组DR = 2.82 +/- 0.39，尿毒症大鼠DR = 2.52 +/- 0.40）。与对照大鼠相比，尿毒症大鼠在缺乏胰岛素的情况下，膈肌和肝脏的AIBA浓度较高。胰岛素有增加对照组大鼠腓肠肌、膈肌和肝脏摄取的预期效果，但对尿毒症大鼠腓肠肌摄取无影响（对照组DR = 5.82 +/- 0.78，尿毒症大鼠DR = 3.07 +/- 0.60）。胰岛素对横膈膜、肝脏及尿毒症大鼠AIBA分布比的影响小于对照组。尿毒症与胰岛素介导的氨基酸摄取抑制以及外周对葡萄糖利用的抵抗有关。
Peripheral tissue resistance to insulin-mediated glucose uptake occurs in uremia. A similar resistance also may exist to insulin-mediated amino acid uptake. The present study examined the effect of insulin on the uptake or distribution ratios (DR) of alpha-aminoisobutyric acid (AIBA) attained in the tissues of rats made acutely uremic by bilateral nephrectomy. Both uremic and control rats concentrated equally AIBA in the gastrocnemius muscle in the absence of insulin (DR = 2.82 +/- 0.39 in controls, 2.52 +/- 0.40 in uremics). Compared to control rats, the diaphragm and liver of uremic rats, in the absence of insulin, had higher concentrations of AIBA. Insulin had the expected effect of increasing the uptake in the gastrocnemius, diaphragm, and liver of control rats but had no effect on uptake in the gastrocnemius muscle of uremic rats (DR = 5.82 +/- 0.78 in controls, 3.07 +/- 0.60 in uremics). The effect of insulin on the distribution ratio of AIBA in diaphragm and liver or uremic rats was less than it was in control rats. Uremia is associated with inhibition of insulin-mediated amino acid uptake as well as peripheral resistance to glucose utilization.