Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids.
Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids.
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DOI：
10.1016/j.xpro.2022.101656
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发表时间：
2022-09-16
期刊：
影响因子：
--
通讯作者：
Sancho, Rocio
中科院分区：
文献类型：
--
作者：
Pedraza-Arevalo, Sergio;Cujba, Ana-Maria;Alvarez-Fallas, Mario Enrique;Sancho, Rocio
人类诱导多能干细胞（hiPSC）和类器官对于体外模拟人类发育和疾病是重要的。在这项研究中，我们描述了一种使hiPSC向胰腺祖细胞（PP）类器官和β样细胞分化的方案。我们详细描述了hiPSC的扩增和接种、PP分化、类器官扩增以及PP分化成β细胞。分化后，类器官含有β、δ和α细胞。有关本方案使用和执行的完整详细信息，请参阅。hiPSC分化为胰腺祖细胞的分步方案在3D类器官中扩增胰腺祖细胞胰腺祖细胞聚集体分化为β样细胞Matrigel圆顶中胰腺祖细胞类器官的β样细胞分化出版商说明：进行任何实验方案都需要遵守当地机构的实验室安全和伦理指南。人类诱导多能干细胞（hiPSC）和类器官对于体外模拟人类发育和疾病是重要的。在这项研究中，我们描述了一种使hiPSC向胰腺祖细胞（PP）类器官和β样细胞分化的方案。我们详细描述了hiPSC的扩增和接种、PP分化、类器官扩增以及PP分化成β细胞。分化后，类器官含有β、δ和α细胞。
Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells. For complete details on the use and execution of this protocol, please refer to. Step-by-step protocol for hiPSC differentiation to pancreatic progenitors Expansion of pancreatic progenitors in 3D organoids Differentiation of pancreas progenitor cell aggregates to beta-like cells Beta-like cell differentiation of pancreatic progenitor organoids in Matrigel domes Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells.
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影响因子：
8.8
作者：
Cujba AM;Alvarez-Fallas ME;Pedraza-Arevalo S;Laddach A;Shepherd MH;Hattersley AT;Watt FM;Sancho R
通讯作者：
Sancho R
增强了人多能干细胞分化为胰腺祖细胞共表达PDX1和NKX6.1。
影响因子：
7.5
作者：
Memon B;Karam M;Al-Khawaga S;Abdelalim EM
通讯作者：
Abdelalim EM
影响因子：
5.9
作者：
Trott J;Tan EK;Ong S;Titmarsh DM;Denil SLIJ;Giam M;Wong CK;Wang J;Shboul M;Eio M;Cooper-White J;Cool SM;Rancati G;Stanton LW;Reversade B;Dunn NR
通讯作者：
Dunn NR
影响因子：
11.4
作者：
Russ, Holger A.;Parent, Audrey V.;Hebrok, Matthias
通讯作者：
Hebrok, Matthias