Antinociceptive effects of dehydrocorydaline in mouse models of inflammatory pain involve the opioid receptor and inflammatory cytokines.
Antinociceptive effects of dehydrocorydaline in mouse models of inflammatory pain involve the opioid receptor and inflammatory cytokines.
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DOI：
10.1038/srep27129
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发表时间：
2016-06-07
影响因子：
4.6
通讯作者：
Gu XP
中科院分区：
文献类型：
--
作者：
Yin ZY;Li L;Chu SS;Sun Q;Ma ZL;Gu XP
脱氢紫堇碱 (DHC) 是从紫堇中分离出来的生物碱成分。此前的研究表明，DHC具有抗炎、抗肿瘤作用，并能保护心血管系统。然而，关于 DHC 体内抗伤害作用的研究很少。本研究使用两种炎性疼痛模型：乙酸诱导的扭体试验和福尔马林爪试验，探讨了 DHC 对小鼠的镇痛作用和可能的机制。腹腔内给予DHC（3.6、6或10mg/kg）在乙酸诱导的扭体试验中显示出剂量依赖性的抗伤害作用，并显着减弱小鼠福尔马林诱导的疼痛反应。 DHC的镇痛作用与动物的运动活动或运动反应的变化无关，并且在小鼠中未观察到明显的急性或慢性毒性作用。此外，纳洛酮的使用证实了阿片受体参与 DHC 的中枢抗伤害作用。 DHC 可减轻福尔马林引起的足爪水肿，这表明 DHC 可能在外周产生抗炎作用。在福尔马林试验中，DHC降低脊髓中半胱天冬酶6（CASP6）、TNF-α、IL-1β和IL-6蛋白的表达。这些发现证实 DHC 对小鼠具有抗伤害作用。
Dehydrocorydaline (DHC) is an alkaloidal component isolated from Rhizoma corydalis. Previous studies have shown that DHC has anti-inflammatory and anti-tumor effects and that it can protect the cardiovascular system. However, there are few studies of the antinociceptive effects of DHC in vivo. This study explored the antinociceptive effects and possible mechanisms of DHC in mice using two inflammatory pain models: the acetic acid-induced writhing test and the formalin paw test. The intraperitoneal administration of DHC (3.6, 6 or 10 mg/kg) showed a dose-dependent antinociceptive effect in the acetic acid-induced writhing test and significantly attenuated the formalin-induced pain responses in mice. The antinociceptive effects of DHC were not associated with changes in the locomotor activity or motor responses of animals, and no obvious acute or chronic toxic effects were observed in the mice. Furthermore, the use of naloxone confirmed the involvement of the opioid receptor in the central antinociceptive effects of DHC. DHC reduced formalin-induced paw edema, which indicated that DHC may produce an anti-inflammatory effect in the periphery. In the formalin test, DHC decreased the expression of caspase 6 (CASP6), TNF-α, IL-1β and IL-6 proteins in the spinal cord. These findings confirm that DHC has antinociceptive effects in mice.