Microarray-based discovery of highly expressed olfactory mucosal genes: potential roles in the various functions of the olfactory system
Microarray-based discovery of highly expressed olfactory mucosal genes: potential roles in the various functions of the olfactory system
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DOI：
10.1152/physiolgenomics.00117.2003
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发表时间：
2003-12-16
影响因子：
4.6
通讯作者：
Aronow, BJ
中科院分区：
文献类型：
--
作者：
Genter, MB;Van Veldhoven, PP;Aronow, BJ
我们试图通过检查相对于其他81个发育中和成年小鼠组织中的过表达基因，获得对嗅粘膜（OM）（成年脊椎动物神经发生和神经再生的主要部位）中组织特异性基因表达的整体看法。我们使用统计学和倍数差异标准的组合来从Incyte小鼠GEM 1阵列上的8，734个小鼠cDNA元件的阵列中鉴定前269个克隆的cDNA。这些克隆，代表已知的和不好表征的基因转录本，根据它们在其他组织中的相对表达模式进行分组，然后进一步检查基因本体论类别。269个基因中约三分之一也在发育和/或成年中枢神经系统组织中高度表达。其中一些已经被建议或证明在神经发生，神经元分化和/或神经元迁移中发挥作用，进一步表明许多共享这种表达模式的未知基因可能发挥类似的作用。高度OM特异性基因包括腭、肺和鼻上皮癌相关基因（Plunc）、磷酸鞘氨醇裂解酶（Sgpl 1）和对氧磷酶1（Pon 1）. OM内的细胞类型特异性表达是通过原位杂交几个代表性的表达模式簇建立的.使用ENSEMBL组装的小鼠基因组和对人类基因组的比较基因组学分析，我们将许多未知的表达序列标签（EST）和表征不佳的基因分配给新的或已知的基因产物，并提供预测性分类。对该数据库的进一步探索将为嗅觉神经发生、神经元分化、嗅觉和粘膜防御的关键基因和途径提供更多的见解。
We sought to gain a global view of tissue- specific gene expression in the olfactory mucosa ( OM), the major site of neurogenesis and neuroregeneration in adult vertebrates, by examination of its overexpressed genes relative to that in 81 other developing and adult mouse tissues. We used a combination of statistical and fold- difference criteria to identify the top 269 cloned cDNAs from an array of 8,734 mouse cDNA elements on the Incyte Mouse GEM1 array. These clones, representing known and poorly characterized gene transcripts, were grouped according to their relative expression patterns across the other tissues and then further examined with respect to gene ontology categories. Approximately one- third of the 269 genes were also highly expressed in developing and/ or adult central nervous system tissues. Several of these have been suggested or demonstrated to play roles in neurogenesis, neuronal differentiation, and/ or neuronal migration, further suggesting that many of the unknown genes that share this expression pattern may play similar roles. Highly OM- specific genes included a palate, lung, and nasal epithelium carcinoma- associated gene ( Plunc); sphingosine phosphate lyase ( Sgpl1), and paraoxonase 1 ( Pon1). Cell- type- specific expression within OM was established using in situ hybridization for several representative expression pattern clusters. Using the ENSEMBL- assembled mouse genome and comparative genomics analyses to the human genome, we assigned many of the unknown expressed sequence tags ( ESTs) and poorly characterized genes to either novel or known gene products and provided predictive classification. Further exploration of this database will provide additional insights into genes and pathways critical for olfactory neurogenesis, neuronal differentiation, olfaction, and mucosal defense.