Dynamic [Cl(-)](i) measurement with chloride sensing quantum dots nanosensor in epithelial cells.
Dynamic [Cl(-)](i) measurement with chloride sensing quantum dots nanosensor in epithelial cells.
复制标题
上皮细胞中氯离子传感量子点纳米传感器的动态 [Cl(-)](i) 测量。
DOI：
10.1088/0957-4484/21/5/055101
复制
发表时间：
2010
期刊：
影响因子：
3.5
通讯作者：
Wong,LidB
中科院分区：
文献类型：
--
作者：
Wang,Yuchi;Mao,Hua;Wong,LidB
我们已经合成了一种氯离子传感量子点（QD）纳米传感器，Cl-QD，用于在毫摩尔范围内动态测量氯离子浓度，这种灵敏度适用于上皮细胞中大多数生理细胞内氯离子浓度（[Cl−] i）的测量。通过将阴离子受体1-（2-巯基-乙基）-3-苯基-硫脲（MEPTU）与水溶性的CdSe/ZnS量子点在620 nm的发射波长处缀合来合成Cl-量子点。在氯离子与Cl-QD结合后，光诱导电子转移机制导致QD的荧光淬灭。这导致氯离子浓度与Cl-QD的荧光强度之间的反比关系。我们已经利用这种Cl-QD测量了T84和CF-PAC培养细胞中的[Cl−] i，其中C1 C-2或CFTR氯离子通道由药理学氯离子通道激活剂和抑制剂操纵。鲁比前列酮和染料木素分别激活T84中的C1 C-2和CFTR氯离子通道，导致Cl-QD荧光的预测性增加，即[Cl−] i降低。相反，将氯离子通道抑制剂格列本脲应用于CF-PAC细胞，由于[Cl−] i增加，导致Cl-QD荧光的可预测降低。这些是使用基于QD的氯离子传感器动态测量上皮细胞中细胞内氯离子浓度的第一个数据。
We have synthesized a chloride sensing quantum dots (QD) nanosensor, Cl-QD, for the dynamic measurements of chloride ion concentration in the millimolar range, a sensitivity that is applicable to most physiological intracellular chloride ion concentration ([Cl−] i) measurements in epithelial cells. The Cl-QD is synthesized by conjugating an anion receptor, 1-(2-mercapto-ethyl)-3-phenyl-thiourea (MEPTU) to a water soluble CdSe/ZnS QD at an emission wavelength of 620 nm. Upon binding of chloride ions to the Cl-QD, a photo-induced electron transfer mechanism caused the fluorescence of the QD to quench. This resulted in an inversely proportional relationship between the chloride ion concentration and the fluorescence intensity of the Cl-QD. We have utilized this Cl-QD to measure [Cl−] i in T84 and CF-PAC cultured cells, with either the C1C-2 or CFTR chloride channels being manipulated by pharmacological chloride channel activators and inhibitors. Activations of C1C-2 and CFTR chloride channels in T84 by the respective lubiprostone and genistein caused predictive increases in the fluorescence of the Cl-QD, ie, a decrease of [Cl−] i. Conversely, glibenclamide, a chloride channel inhibitor, applied to the CF-PAC cells caused a predictable decrease in the fluorescence of Cl-QD due to the increase of [Cl−] i. These are the first data in using QD-based chloride ion sensors for dynamic measurements of intracellular chloride ion concentrations in epithelial cells.