Phylogenetic Analysis of Harmful Algal Bloom (HAB)-Causing Dinoflagellates Along the Korean Coasts, Based on SSU rRNA Gene
Phylogenetic Analysis of Harmful Algal Bloom (HAB)-Causing Dinoflagellates Along the Korean Coasts, Based on SSU rRNA Gene
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基于 SSU rRNA 基因对韩国海岸引起有害藻华 (HAB) 的甲藻进行系统发育分析
DOI：
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发表时间：
2004
影响因子：
2.8
通讯作者：
Jung
中科院分区：
文献类型：
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作者：
Se Hee Kim;Keun;Chang;W. Lee;Man Chang;Jung
从韩国沿海水域分离出23株有害藻华(HAB)-（引起鞭毛藻）的培养物。对14种形态种中的每一种进行核arencoded small subunit (SSU) rDNA分析，以确定物种的系统发育亲缘性。在时空隔离条件下，3-4个克隆培养株的SSU rDNA序列完全相同。赤血菌和多鼓菌的SSU rDNA序列存在异质性。在一株绢绢海Al. tamarense克隆培养中，SSU rRNA基因显示出极端序列多态性。在GenBank中进行同源性检索，发现11种鞭毛纲动物按其形态分类聚在一起。本研究首次测定到的聚核蠓、纹尾蠓和鹿角蠓的SSU rDNA序列表明：聚核蠓形成了一个独立的分支，与其他鞭毛类分离；Gyr。纹状体与Gyr组为单系组。背和Gyr。uncatenum;phh hartmanii属裸子藓属（Gymnodinium sensu Hansen）和Moestrup属。
Twenty-three cultures of harmful algal bloom (HAB)-(causing dinoflagellates were isolated from the coastal waters of Korea. For each of the 14 morphospecies, the nuclearencoded small subunit (SSU) rDNA was analyzed to determine the phylogenetic relatedness of the species. Despite temporal and spatial isolation, 3-4 clonal cultures of Alexandrium catenella, Cochlodinium polykrikoides, and Gymnodinium catenatum had 100% identical SSU rDNA sequences. In contrast, heterogeneities in the SSU rDNA sequences were observed in Akashiwo sanguinea and Lingulodinium polyedrum strains. Extreme sequence polymorphism was shown within the SSU rRNA genes of an Al. tamarense clonal culture. A homology search in GenBank revealed that 11 dinoflagellate species were located in clusters corresponding to their morphological classification. The SSU rDNA sequences of C. polykrikoides, Gyrodinium instriatum, and Pheopolykrikos hartmannii, which were determined for the first time in this study, showed the following phylogenetic relationships: C. polykrikoides formed an independent branch separated from other dinoflagellates; Gyr. instriatum was placed in a monophyletic group with Gyr. dorsum and Gyr. uncatenum; and Ph. hartmanii, which forms a distinct two-celled pseudocolony, belonged to Gymnodinium sensu Hansen and Moestrup.