Investigating the potential use of environmental DNA (eDNA) for genetic monitoring of marine mammals.
Investigating the potential use of environmental DNA (eDNA) for genetic monitoring of marine mammals.
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调查环境 DNA (eDNA) 在海洋哺乳动物遗传监测中的潜在用途。
DOI：
10.1371/journal.pone.0041781
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gilbert MT
中科院分区：
文献类型：
--
作者：
Foote AD;Thomsen PF;Sveegaard S;Wahlberg M;Kielgast J;Kyhn LA;Salling AB;Galatius A;Orlando L;Gilbert MT
非侵入性样品的开发已广泛应用于陆地物种的遗传监测。在水生生态系统中，粪便、脱落的毛发或皮肤等非侵入性样本不易获得。然而，环境DNA（eDNA）的使用最近被证明是一个有效的工具，在淡水生态系统中的物种存在的遗传监测。由于与大多数淡水生态系统相比，海洋环境中的稀释度、混合量和盐度更大，使用eDNA检测海洋环境中的物种可能面临更大的挑战。为了确定eDNA用于遗传监测的潜在用途，我们使用了扩增短线粒体DNA序列的特异性引物，以检测在受控环境和自然海洋位置中海洋哺乳动物，港湾鼠海豚，Phocoena phocoena的存在。通过与静态声学监测装置检测到的港湾江豚回声定位喀喇声进行比较，考察了基因检测的可靠性。虽然我们能够在受控条件下一致地对目标物种进行遗传检测，但自然位置的结果不太一致，并且eDNA检测不如声学检测成功。然而，在一个地点，我们发现了长鳍领航鲸，Globicephala melas，这是一种在波罗的海很少看到的物种。因此，优化旨在处理更大体积的海水，这种方法有可能补充目前的视觉和声学方法的海洋哺乳动物的物种检测。
The exploitation of non-invasive samples has been widely used in genetic monitoring of terrestrial species. In aquatic ecosystems, non-invasive samples such as feces, shed hair or skin, are less accessible. However, the use of environmental DNA (eDNA) has recently been shown to be an effective tool for genetic monitoring of species presence in freshwater ecosystems. Detecting species in the marine environment using eDNA potentially offers a greater challenge due to the greater dilution, amount of mixing and salinity compared with most freshwater ecosystems. To determine the potential use of eDNA for genetic monitoring we used specific primers that amplify short mitochondrial DNA sequences to detect the presence of a marine mammal, the harbor porpoise, Phocoena phocoena, in a controlled environment and in natural marine locations. The reliability of the genetic detections was investigated by comparing with detections of harbor porpoise echolocation clicks by static acoustic monitoring devices. While we were able to consistently genetically detect the target species under controlled conditions, the results from natural locations were less consistent and detection by eDNA was less successful than acoustic detections. However, at one site we detected long-finned pilot whale, Globicephala melas, a species rarely sighted in the Baltic. Therefore, with optimization aimed towards processing larger volumes of seawater this method has the potential to compliment current visual and acoustic methods of species detection of marine mammals.
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影响因子：
56.9
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通讯作者：
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通讯作者：
Venter JC