The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
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DOI：
10.1038/36786
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发表时间：
1997-11-20
期刊：
影响因子：
64.8
通讯作者：
Danchin, A
中科院分区：
文献类型：
--
作者：
Kunst, F;Ogasawara, N;Danchin, A
枯草芽孢杆菌是革兰氏阳性菌中最具特征的成员。它的基因组由4,214,810个碱基对组成，包括4,100个蛋白质编码基因。在这些编码蛋白质的基因中，53%只出现一次，而基因组的四分之一对应于几个基因家族，这些基因家族通过基因复制而大大扩大，最大的家族包含77个假定的ATP结合运输蛋白。此外，遗传能力的很大一部分用于各种碳源的利用，包括许多植物衍生的分子。考虑到芽孢杆菌菌株分泌大量工业重要酶的能力，识别五个信号肽基因以及分泌装置组件的几个基因是重要的。许多基因与包括抗生素在内的次生代谢物的合成有关，而抗生素更多地与链霉菌有关。基因组中至少含有10个前驱体或前驱体残留物，表明噬菌体感染在水平基因转移，特别是在细菌致病的繁殖中发挥了重要的进化作用。
Bacillus subtilis Is the best-characterized member of the Gram-positive bacteria. Its genome of 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these protein-coding genes, 53% are represented once, while a quarter of the genome corresponds to several gene families that have been greatly expanded by gene duplication, the largest family containing 77 putative ATP-binding transport proteins. In addition, a large proportion of the genetic capacity is devoted to the utilization of a variety of carbon sources, including many plant-derived molecules. The identification of five signal peptidase genes, as well as several genes for components of the secretion apparatus, is important given the capacity of Bacillus strains to secrete large amounts of industrially important enzymes. Many of the genes are involved in the synthesis of secondary metabolites, Including antibiotics, that are more typically associated with Streptomyces species. The genome contains at least ten prophages or remnants of prophages, indicating that bacteriophage Infection has played an important evolutionary role in horizontal gene transfer, in particular In the propagation of bacterial pathogenesis.