Leader RNA regulates snakehead vesiculovirus replication via interacting with viral nucleoprotein
Leader RNA regulates snakehead vesiculovirus replication via interacting with viral nucleoprotein
复制标题
DOI：
10.1080/15476286.2020.1818960
复制
发表时间：
2020-09
期刊：
影响因子：
4.1
通讯作者：
Xiangmou Qin;Shuangshuang Feng;Yanwei Zhang;Jianguo Su;Li Lin;Yong-An Zhang;Jiagang Tu
中科院分区：
文献类型：
--
作者：
Xiangmou Qin;Shuangshuang Feng;Yanwei Zhang;Jianguo Su;Li Lin;Yong-An Zhang;Jiagang Tu
摘要前导RNA是一种来源于病毒的小分子非编码RNA，在病毒复制过程中起着重要的调控作用，但其作用机制尚不清楚。本研究以引起我国养殖乌鳢高死亡率的鱼类弹状病毒--乌鳢水泡病毒（snakeheadvesiculovirus，SHVV）为研究对象，旨在揭示其前导RNA的分子功能。SHVV感染细胞的高通量小RNA测序表明，SHVV在感染过程中产生两组前导RNA（命名为legroup 1和legroup 2）。过表达和敲除实验表明，legroup 1，而不是legroup 2，影响SHVV复制。从机制上讲，legroup 1介导的SHVV复制调控与其与病毒核蛋白（N）的相互作用有关。此外，还发现legroup 1的第6-10位核苷酸是与N蛋白相互作用的关键区域，而N蛋白的第1-45位氨基酸则是与legroup 1相互作用的关键区域。总之，我们确定了两组SHVV前导RNA，并揭示了两种类型的前导RNA之一在病毒复制中的作用。本研究将有助于了解前导RNA在负链RNA病毒复制调控中的作用。
ABSTRACT Leader RNA, a kind of virus-derived small noncoding RNA, has been proposed to play an important role in regulating virus replication, but the underlying mechanism remains elusive. In this study, snakehead vesiculovirus (SHVV), a kind of fish rhabdovirus causing high mortality to the cultured snakehead fish in China, was used to unveil the molecular function of leader RNA. High-throughput small RNA sequencing of SHVV-infected cells showed that SHVV produced two groups of leader RNAs (named legroup1 and legroup2) during infection. Overexpression and knockout experiments reveal that legroup1, but not legroup2, affects SHVV replication. Mechanistically, legroup1-mediated regulation of SHVV replication was associated with its interaction with the viral nucleoprotein (N). Moreover, the nucleotides 6–10 of legroup1 were identified as the critical region for its interaction with the N protein, and the amino acids 1–45 of N protein were proved to confer its interaction with the legroup1. Taken together, we identified two groups of SHVV leader RNAs and revealed a role in virus replication for one of the two types of leader RNAs. This study will help understand the role of leader RNA in regulating the replication of negative-stranded RNA viruses.